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Splicing function of mitotic regulators links R-loop mediated DNA damage to tumor cell killing

ABSTRACT: We discovered that two mitotic regulators, BuGZ and Bub3, involved in splicing regulation during interphase 8 samples from primary Human foreskin fibroblast cells (HFFs) , 12 samples from TOV21G cells. Control siRNA. BuGZ siRNA or Bub3 siRNA were transfected for 48 h before sample collection. Cells treated with pladienolide B served as positive controls. For each RNAi experiment, we had two biological replicates.

ORGANISM(S): Homo sapiens

SUBMITTER: Yixian Zheng

PROVIDER: E-GEOD-67770 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Splicing function of mitotic regulators links R-loop-mediated DNA damage to tumor cell killing.

Wan Yihan Y Zheng Xiaobin X Chen Haiyang H Guo Yuxuan Y Jiang Hao H He Xiaonan X Zhu Xueliang X Zheng Yixian Y

The Journal of cell biology 20150401 2

Although studies suggest that perturbing mitotic progression leads to DNA damage and p53 activation, which in turn lead to either cell apoptosis or senescence, it remains unclear how mitotic defects trigger p53 activation. We show that BuGZ and Bub3, which are two mitotic regulators localized in the interphase nucleus, interact with the splicing machinery and are required for pre-mRNA splicing. Similar to inhibition of RNA splicing by pladienolide B, depletion of either BuGZ or Bub3 led to incre ...[more]

PMID: 25918225

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