Proteomics

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Proteomic analysis of ARPE-19 cells exposed to chronic H2O2 treatment

ABSTRACT: We performed a proteomic analysis of a retinal pigment epithelium cell line (ARPE-19) exposed to long-term sublethal levels of H2O2 to mimic low-level pro-oxidative insult in age-related macular degeneration (AMD). using a wide range of biochemical analyses and cell culture techniques, we highlighted changes in energy metabolism, extracellular matrix organization and inflammation-related processes, which may help in understanding the molecular pathogenesis of AMD.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Retinal Pigment Epithelial Cell, Cell Culture

DISEASE(S): Age Related Macular Degeneration

SUBMITTER: Davide Chiasserini  

LAB HEAD: Davide Chiasserini

PROVIDER: PXD022545 | Pride | 2021-01-04

REPOSITORIES: Pride

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2020-07-14_MS_PG_Proteome_Davide_Casserini_CTRL_01_20200717061234.raw Raw
2020-07-14_MS_PG_Proteome_Davide_Casserini_CTRL_02_20200717083719.raw Raw
2020-07-14_MS_PG_Proteome_Davide_Casserini_CTRL_03_20200717110239.raw Raw
2020-07-14_MS_PG_Proteome_Davide_Casserini_H2O2_01.raw Raw
2020-07-14_MS_PG_Proteome_Davide_Casserini_H2O2_02.raw Raw
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Publications

Crosstalk between Long-Term Sublethal Oxidative Stress and Detrimental Inflammation as Potential Drivers for Age-Related Retinal Degeneration.

Macchioni Lara L   Chiasserini Davide D   Mezzasoma Letizia L   Davidescu Magdalena M   Orvietani Pier Luigi PL   Fettucciari Katia K   Salviati Leonardo L   Cellini Barbara B   Bellezza Ilaria I  

Antioxidants (Basel, Switzerland) 20201229 1

Age-related retinal degenerations, including age-related macular degeneration (AMD), are caused by the loss of retinal pigmented epithelial (RPE) cells and photoreceptors. The pathogenesis of AMD, deeply linked to the aging process, also involves oxidative stress and inflammatory responses. However, the molecular mechanisms contributing to the shift from healthy aging to AMD are still poorly understood. Since RPE cells in the retina are chronically exposed to a pro-oxidant microenvironment throu  ...[more]

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