Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Comparison of transcriptomes in class-B GATA mutants with cytokinin treatments

ABSTRACT: We have used Agilent whole genome arrays (v4) to compare the gene expression changes between 14 days-old Arabidopsis seedlings with or without cytokinin treatments in wild type as well as the following mutant genotypes: gnc gnl (AT5G56860, AT4G26150), gata16 gata17 gata17l (AT5G49300, AT3G16870, AT4G16141), gnc gnl gata16 gata17 gata17l (AT5G56860, AT4G26150, AT5G49300, AT3G16870, AT4G16141) Three independent biological samples were prepared for each genotype. Experiment was performed in two rounds: Col-0_1, gnc gnl, quintuple and Col-0_2, triple. Three replicate measurements of three biological samples were prepared for each genotype.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Quirin Ranftl

PROVIDER: E-GEOD-71828 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

LLM-Domain Containing B-GATA Factors Control Different Aspects of Cytokinin-Regulated Development in Arabidopsis thaliana.

Ranftl Quirin L QL Bastakis Emmanouil E Klermund Carina C Schwechheimer Claus C

Plant physiology 20160201 4

Leu-Leu-Met (LLM)-domain B-GATAs are a subfamily of the 30-membered GATA transcription factor family from Arabidopsis. Only two of the six Arabidopsis LLM-domain B-GATAs, i.e. GATA, NITRATE-INDUCIBLE, CARBON METABOLISM-INVOLVED (GNC) and its paralog GNC-LIKE/CYTOKININ-RESPONSIVE GATA FACTOR1 (GNL), have already been analyzed with regard to their biological function. Together, GNC and GNL control germination, greening, flowering time, and senescence downstream from auxin, cytokinin (CK), gibberel ...[more]

PMID: 26829982

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Project description:Jatropha curcas L. is a potential biofuel plant. Application of exogenous cytokinin (6-benzyladenine, BA) on its inflorescence buds can significantly increase the number of female flowers, thereby improving seed yield. To investigate which genes and signal pathways are involved in the response to cytokinin in J. curcas inflorescence buds, we monitored transcriptional activity in inflorescences at 0, 3, 12, 24, and 48 h after BA treatment using a microarray. We detected 5,555 differentially expressed transcripts over the course of the experiment, which could be grouped into 12 distinct temporal expression patterns. We also identified 31 and 131 transcripts in J. curcas whose homologs in model plants function in flowering and phytohormonal signaling pathways, respectively. According to the transcriptional analysis of genes involved in flower development, we hypothesized that BA treatment delays floral organ formation by inhibiting the transcription of the A, B and E classes of floral organ-identity genes, which would allow more time to generate more floral primordia in inflorescence meristems, thereby enhancing inflorescence branching and significantly increasing flower number per inflorescence. BA treatment might also play an important role in maintaining the flowering signals by activating the transcription of GIGANTEA (GI) and inactivating the transcription of CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) and TERMINAL FLOWER 1b (TFL1b). In addition, exogenous cytokinin treatment could regulate the expression of genes involved in the metabolism and signaling of other phytohormones, indicating that cytokinin and other phytohormones jointly regulate flower development in J. curcas inflorescence buds. Our study provides a framework to better understand the molecular mechanisms underlying changes in flowering traits in response to cytokinin treatment in J. curcas inflorescence buds. The results provide valuable information related to the mechanisms of cross-talk among multiple phytohormone signaling pathways in woody plants. Gene expression of flower buds in response to BA was measured at 0h, 3h, 12h, 24h and 48h after 1mM 6-benzylaminopurine treatment. Three biological replicates were prepared for each time point.

Dataset Information

Comparison of transcriptomes in class-B GATA mutants with cytokinin treatments

Publications

LLM-Domain Containing B-GATA Factors Control Different Aspects of Cytokinin-Regulated Development in Arabidopsis thaliana.

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