Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

32 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of miRNAs and its targets using high-throughput sequencing and degradome analysis in male and female Asparagus officinalis.

ABSTRACT: Two independent small RNA (sRNA) libraries from male and female asparagus plants were sequenced, generating 4.13 and 5.88 million final cleaned reads, respectively. A total of 154 conserved miRNA belonging to 26 families, and 40 novel miRNA candidates that seemed to be specific to asparagus were identified, among them, 63 miRNAs exhibited significant differential expression between male and female plants, and 36 target mRNAs representing 44 conserved and fournovel miRNA in asparagus by high-throughput degradome sequencing analysis. Examination of small RNA in male and female asparagus using high-throughput sequencing

ORGANISM(S): Asparagus officinalis

SUBMITTER: Li Qin

PROVIDER: E-GEOD-72594 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Identification of miRNAs and its targets using high-throughput sequencing and degradome analysis in male and female Asparagus officinalis.

Project description:Two independent small RNA (sRNA) libraries from male and female asparagus plants were sequenced, generating 4.13 and 5.88 million final cleaned reads, respectively. A total of 154 conserved miRNA belonging to 26 families, and 40 novel miRNA candidates that seemed to be specific to asparagus were identified, among them, 63 miRNAs exhibited significant differential expression between male and female plants, and 36 target mRNAs representing 44 conserved and fournovel miRNA in asparagus by high-throughput degradome sequencing analysis.

2015-09-02 | GSE72594 | GEO

The role of linear ubiquitination in memory formation

Project description:This project examined the role of linear polyubiquitination in the amygdala during fear memory formation in male and female rats.

2021-06-28 | PXD025483 | Pride

Identification and characterization of microRNA expression in Ginkgo biloba L. leaves

Project description:High-throughput small RNA sequencing were performed to identify a large number of miRNAs and their targets in mature female and male G. biloba leaves for the first time. We ascertained that the regulatory networks of the miRNAs are involved in many different primary biological processes based on potential target designations. Our study is the first to provide useful information for uncovering the regulatory networks of miRNAs in basal gymnosperm G. biloba leaves. small RNA sequencing in female and male leaves of G. biloba

2015-07-13 | E-GEOD-65229 | biostudies-arrayexpress

Proteome analysis of male accessory gland secretions in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)

Project description:In insects, male accessory gland proteins (ACPs) are important reproductive proteins secreted by male accessory glands (MAGs) of the internal male reproductive system. During mating, ACPs were transferred along with sperms inside female bodies and have a significant impact on the physiology of female reproduction. Under sexual selection pressures, the ACPs exhibit remarkably rapid and divergent evolution and varies from species to species. The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a major insect pest of cruciferous vegetables worldwide. The reproductive physiology on post-mating state of this species is still largely unknown, which is important for management of this pest. In this study, the ACPs transferred into females during mating were identified by using a tandem mass tags quantitative proteomic analysis. The MAGs were compared before and after mating immediately. In total, we identified 123 putative secreted ACPs, including most important physiological: regulators of proteolysis, transporters and protein export machinery, signal transduction and immunity. Comparing P. xylostella with other four insect ACPs, trypsins were the only ACPs detected in all insect species. This was the first time to identify and analyze ACPs in P. xylostella. Our results have provided an important list of putative secreted ACPs, and have set the stage for further explore functions of these putative proteins in P. xylostella reproduction.

2023-03-11 | PXD038931 | Pride

Proteome analysis of male accessory gland secretions in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)

Project description:In insects, male accessory gland proteins (ACPs) are important reproductive proteins secreted by male accessory glands (MAGs) of the internal male reproductive system. During mating, ACPs were transferred along with sperms inside female bodies and have a significant impact on the physiology of female reproduction. Under sexual selection pressures, the ACPs exhibit remarkably rapid and divergent evolution and varies from species to species. The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a major insect pest of cruciferous vegetables worldwide. The reproductive physiology on post-mating state of this species is still largely unknown, which is important for management of this pest. In this study, the ACPs transferred into females during mating were identified by using a tandem mass tags quantitative proteomic analysis. The MAGs were compared before and after mating immediately. The proteomes of copulatory bursas (CB) in mated females shortly after mating were also analyzed by shotgun LC-MS/MS technique. In total, we identified 123 putative secreted ACPs, including most important physiological: regulators of proteolysis, transporters and protein export machinery, signal transduction and immunity. Comparing P. xylostella with other four insect ACPs, trypsins were the only ACPs detected in all insect species. We also identified some new insect ACPs, including protein with chitin binding Peritrophin-A domain, PMP-22/EMP/MP20/Claudin tight junction domain-containing protein, netrin-1, type II inositol 1,4,5-trisphosphate 5-phosphatase, two spaetzles, allatostatin-CC and cuticular protein. This was the first time to identify and analyze ACPs in P. xylostella. Our results have provided an important list of putative secreted ACPs, and have set the stage for further explore functions of these putative proteins in P. xylostella reproduction.

2023-03-11 | PXD039074 | Pride

Drosophila melanogaster small RNA sequence from 14 libraries

Project description:High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Small RNAs were sequenced from D. melanogaster heads (male and female), body (male and female), S2 and Kc cells and different stages of embryo. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt

2008-06-01 | E-GEOD-11624 | biostudies-arrayexpress

Sex differences in the role of protein sumoylation in the amygdala during fear memory formation

Project description:This project examined sex differences in the role of protein sumoylation in the amygdala during context fear memory formation in male and female rats.

2022-05-27 | PXD029825 | Pride

Sex differences in the role of protein degradation in the hippocampus during memory formation

Project description:This project examined sex differences in the role of degradation-specific protein polyubiquitination in the hippocampus during context fear memory formation in male and female rats.

2021-06-28 | PXD026212 | Pride

Sex differences in the role of protein degradation in the amygdala during fear memory formation

Project description:This project examined sex differences in the role of degradation-specific protein polyubiquitination in the amygdala during context fear memory formation in male and female rats.

2021-09-28 | PXD027544 | Pride

Sex differences in the role of K63 polyubiquitination in the amygdala during fear memory formation

Project description:This project examined sex differences in the role of K63 polyubiquitination in the amygdala during context fear memory formation in male and female rats.

2023-05-18 | PXD036369 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data