Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Impact of Arylsulfatase B (ARSB) on gene expression

ABSTRACT: Arylsulfatase B (ARSB; N-acetylgalactosamine-4-sulfatase) null mouse hepatic gene expression was compared with control C57BL/6J mice To determine if there were differences in gene expression between ARSB null mice and matched control mice. ARSB is the enzyme that removes 4-sulfate groups from chondroitin 4-sulfate and dermatan sulfate and thereby regulates their degradation. comparison between ARSB-null mice on C57BL/6J background and control C57BL/6J mice matched for age and gender

ORGANISM(S): Mus musculus

SUBMITTER: Zarema Arbieva

PROVIDER: E-GEOD-77689 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Similar Datasets

Project description:Sulfate-reducing bacteria (SRB) colonize the guts of ~50% of humans. We used genome-wide transposon mutagenesis and insertion-site sequencing (INSeq), RNA-Seq, plus mass spectrometry to characterize genetic and environmental factors that impact the niche of Desulfovibrio piger, the most common SRB in a surveyed cohort of healthy USA adults. Gnotobiotic mice were colonized with an assemblage of sequenced human gut bacterial species with or without D. piger and fed diets with different levels and types of carbohydrates and sulfur sources. Diet was a major determinant of functions expressed by this artificial 9-member community and of the genes that impact D. piger fitness; the latter includes high- and low-affinity systems for utilizing ammonia, a limiting resource for D. piger in mice consuming a polysaccharide-rich diet. While genes involved in hydrogen consumption and sulfate reduction are necessary for its colonization, varying dietary free sulfate levels did not significantly alter levels of D. piger, which can obtain sulfate from the host in part via cross-feeding mediated by Bacteroides-encoded sulfatases. Chondroitin sulfate, a common dietary supplement, increased D. piger and H2S levels without compromising gut barrier integrity. A chondroitin sulfate-supplemented diet together with D. piger impacted the assemblage’s substrate utilization preferences, allowing consumption of more reduced carbon sources, and increasing the abundance of the H2-producing Actinobacterium, Collinsella aerofaciens. Our findings provide genetic and metabolic details of how this H2-consuming SRB shapes the responses of a microbiota to diet ingredients, and a framework for examining how individuals lacking D. piger differ from those that harbor it. 8 samples total, 2 gropus of 4 mice: Proximal colon gene expression profiles of gnotobiotic mice colonized with an artificial gut community composed of 8 human gut species (group 1: NoDp) and from mice colonized with the same community plus D. piger (Dp). Mice were fed a HF/HS diet supplemented with 3% chondroitin sulfate. Animals were sacrificed 2 weeks after colonization

Dataset Information

Impact of Arylsulfatase B (ARSB) on gene expression

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets