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MRNA cap methylation in pluripotency and differentiation

ABSTRACT: The mRNA cap recruits factors essential for transcript processing and translation initiation. We report that regulated mRNA cap methylation is a feature of embryonic stem cell (ESC) differentiation. Expression of the mRNA cap methyltransferase activating subunit, RAM, is elevated in ESCs resulting in high levels of mRNA cap methylation and expression of Oct4 and Sox2 and other pluripotency-associated factors. During neural differentiation, RAM is suppressed which is required for loss of Oct4 and Sox2 and correct expression of neural markers. Cells were treated with control or RAM siRNA and cytosolic RNA or polysome RNA (actively translated) was sequenced.

ORGANISM(S): Mus musculus

SUBMITTER: Victoria Cowling

PROVIDER: E-GEOD-81849 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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mRNA Cap Methylation in Pluripotency and Differentiation.

Grasso Laura L Suska Olga O Davidson Lindsay L Gonatopoulos-Pournatzis Thomas T Williamson Ritchie R Wasmus Lize L Wiedlich Simone S Peggie Mark M Stavridis Marios P MP Cowling Victoria H VH

Cell reports 20160721 5

The mRNA cap recruits factors essential for transcript processing and translation initiation. We report that regulated mRNA cap methylation is a feature of embryonic stem cell (ESC) differentiation. Expression of the mRNA cap methyltransferase activating subunit RAM is elevated in ESCs, resulting in high levels of mRNA cap methylation and expression of a cohort of pluripotency-associated genes. During neural differentiation, RAM is suppressed, resulting in repression of pluripotency-associated f ...[more]

PMID: 27452456

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