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Genes in nonpermissive temperature-induced cell differentiation of testicular Sertoli TTE3 cells

ABSTRACT: We performed global scale microarray analysis to identify detailed mechanisms by which nonpermissive temperature induces cell differentiation in testicular Sertoli TTE3 cells harboring temperature-sensitive SV40 large T-antigen by using an Affymetrix GeneChip system. Testicular Sertoli TTE3 cells used in the present study were derived from transgenic mice harboring a temperature-sensitive simian virus 40 large T-antigen. In the TTE3 cells, inactivation of the T-antigen by a nonpermissive temperature at 39˚C led to cell differentiation accompanying elevation of transferrin and cyclin-dependent kinase inhibitor CDKN1A. Of the 22, 690 probe sets analyzed, nonpermissive temperature up-regulated 729 probe sets and down-regulated 471 probe sets by >2.0-fold. Keywords: Sertoli cell; differentiation; temperature-sensitive SV40 large T-antigen; nonpermissive temperature

ORGANISM(S): Mus musculus

PROVIDER: GSE6961 | GEO | 2007/02/23

SECONDARY ACCESSION(S): PRJNA99323

REPOSITORIES: GEO

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Mus musculus

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