Transcription profiling of mouse testicular Sertoli TTE3 cells with SV40 large T antigent to investigate denes in nonpermissive temperature-induced cell differentiation of testicular Sertoli TTE3 cells

ABSTRACT: We performed global scale microarray analysis to identify detailed mechanisms by which nonpermissive temperature induces cell differentiation in testicular Sertoli TTE3 cells harboring temperature-sensitive SV40 large T-antigen by using an Affymetrix GeneChip system. Testicular Sertoli TTE3 cells used in the present study were derived from transgenic mice harboring a temperature-sensitive simian virus 40 large T-antigen. In the TTE3 cells, inactivation of the T-antigen by a nonpermissive temperature at 39˚C led to cell differentiation accompanying elevation of transferrin and cyclin-dependent kinase inhibitor CDKN1A. Of the 22, 690 probe sets analyzed, nonpermissive temperature up-regulated 729 probe sets and down-regulated 471 probe sets by >2.0-fold. Experiment Overall Design: TTE3 cells were cultured at 39˚C for 0, 6, 12, and 24 h. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChip® system with the Mouse Expression Array 430A which was spotted with 22,690 probe sets. Sample preparation for array hybridization was carried out as described in the manufacture’s instructions.

ORGANISM(S): Mus musculus

SUBMITTER: Yoshiaki Tabuchi 

PROVIDER: E-GEOD-6961 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress