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Genes regulated by T cell and B cell derived TNF in naive lymph nodes

ABSTRACT: We present a RNA microarray analysis of RNA`s extracted from naive mesenterial lymph nodes of mice with specific inactivation of tumor necrosis factor only in B cells (B-TNf mice) or in T- and B-cells (TB-TNF mice). Several genes are cooperatively regulated by T cell derived and B cell derived TNF Keywords: cell type comparison design For 1 independent sample 3 mice of one genotype have been sacrificed, mesenterial lymph nodes were isolated and pooled together. Lymph nodes were immediately frozen in luquid N2 and RNA was prepared using Tri-reagent. RNA was precipitated with isopropanol, washed with 70% ethanol and dissolved in DEPC dH2O.

ORGANISM(S): Mus musculus

SUBMITTER: Sergei Grivennikov

PROVIDER: E-GEOD-8610 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cellular source and molecular form of TNF specify its distinct functions in organization of secondary lymphoid organs.

Tumanov Alexei V AV Grivennikov Sergei I SI Kruglov Andrei A AA Shebzukhov Yuriy V YV Koroleva Ekaterina P EP Piao Yulan Y Cui Chang-Yi CY Kuprash Dmitry V DV Nedospasov Sergei A SA

Blood 20100715 18

Secondary lymphoid organs provide a unique microenvironment for generation of immune responses. Using a cell type-specific conditional knockout approach, we have dissected contributions of tumor necrosis factor (TNF) produced by B cells (B-TNF) or T cells (T-TNF) to the genesis and homeostatic organization of secondary lymphoid organs. In spleen, lymph nodes and Peyer patches, the cellular source of TNF, and its molecular form (soluble versus membrane-bound) appeared distinct. In spleen, in addi ...[more]

PMID: 20634375

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Dataset Information

Genes regulated by T cell and B cell derived TNF in naive lymph nodes

Publications

Cellular source and molecular form of TNF specify its distinct functions in organization of secondary lymphoid organs.

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