Unknown,Transcriptomics,Genomics,Proteomics

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EDL933 in human macrophage THP-1 cells at different time point

ABSTRACT: Effect of human macrophages phagocytosis on the global transcriptome of EDL933 at different time. Infection of THP-1 with EDL933. Application of SCOTS technique to have cDNA. Hybridation on microarray. Reference: EDL933 in RPMI medium Keywords: stres response, host-bacteria response, time-course For each time point, 2 microarray are analysed and on each microarray there is 2 replicate Total of 4 replicate for each time point (including reference medium)

ORGANISM(S): Escherichia coli

SUBMITTER: Sébastien Faucher 

PROVIDER: E-GEOD-9671 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Escherichia coli O157:H7 survives within human macrophages: global gene expression profile and involvement of the Shiga toxins.

Poirier Katherine K   Faucher Sébastien P SP   Béland Maxime M   Brousseau Roland R   Gannon Victor V   Martin Christine C   Harel Josée J   Daigle France F  

Infection and immunity 20080825 11

Escherichia coli O157:H7 is an important food-borne pathogen that specifically binds to the follicle-associated epithelium in the intestine, which rapidly brings this bacterial pathogen in contact with underlying human macrophages. Very little information is available about the interaction between E. coli O157:H7 and human macrophages. We evaluated the uptake and survival of strain EDL933 during infection of human macrophages. Surprisingly, EDL933 survived and multiplied in human macrophages at  ...[more]

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