Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Profiling of Dolphin Skin Cells

ABSTRACT: 3 technical replicates from Wild Dolphin PBL Keywords: Immune and stress response analysis Analyzed 3 arrays with a 3 replicate series.

ORGANISM(S): Tursiops truncatus

SUBMITTER: MarineGenomics Project

PROVIDER: E-GEOD-4580 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

A dolphin peripheral blood leukocyte cDNA microarray for studies of immune function and stress reactions.

Mancia Annalaura A Lundqvist Mats L ML Romano Tracy A TA Peden-Adams Margie M MM Fair Patricia A PA Kindy Mark S MS Ellis Blake C BC Gattoni-Celli Sebastiano S McKillen David J DJ Trent Harold F HF Chen Yian Ann YA Almeida Jonas S JS Gross Paul S PS Chapman Robert W RW Warr Gregory W GW

Developmental and comparative immunology 20060920 5

A microarray focused on stress response and immune function genes of the bottlenosed dolphin has been developed. Random expressed sequence tags (ESTs) were isolated and sequenced from two dolphin peripheral blood leukocyte (PBL) cDNA libraries biased towards T- and B-cell gene expression by stimulation with IL-2 and LPS, respectively. A total of 2784 clones were sequenced and contig analysis yielded 1343 unigenes (archived and annotated at ). In addition, 52 dolphin genes known to be important i ...[more]

PMID: 17084893

Similar Datasets

Profiling of WSSV infection in hepatopancreas of shrimp

Project description:Profiles of gene expression in hepatopancreas isolated from shrimp experimentally infected with White Spot Syndrome Virus were compared to those of un-infected controls Keywords: response to viral disease Two groups of eight shrimp were compared in terms of hepatopancreas gene expression, 40 hours after challenge with White Spot Syndrome Virus

2010-07-01 | E-GEOD-4955 | biostudies-arrayexpress

Evaluation of technical (array-to-array) reproducibility of L. vannamei microarray

Project description:Five aliquots of the same RNA sample were labeled and hybridized in parallel to the L. vannamei microarray, in order to assess the technical reproducibility of the tool Keywords: technical validation A sample of gill total RNA was divided in 5 identical aliquots, and each aliquot was labeled and hybridized to an array. The co-relation among the global expression profiles obtained was assessed.

2010-07-01 | E-GEOD-4954 | biostudies-arrayexpress

Crassostrea array characterization

Project description:Determination of tissue-specific expression profiles and heterologous hybridization by multiple Crassostrea spp. Keywords: Gill versus Hepatopancreas 3 replicate hybridizations, tissue pooled from three individuals

2010-07-01 | E-GEOD-5293 | biostudies-arrayexpress

Tissue-specific expression profiles in L. vannamei

Project description:RNA from different tissues of Litopenaeus vannamei was used to test the ability of the microarray to discriminate tissue-specific gene expression profiles Keywords: validation, tissue comparison RNA from circulating hemocytes, gills, hepatopancreas, or muscle (3 biological replicates each) was hybridized to the L. vannamei microarray. The number of hybridizing genes was noted and the relationships among the global profiles in each sample were determined by cluster analysis

2010-07-01 | E-GEOD-4949 | biostudies-arrayexpress

Gene expression in white adipose tissue stimulated by beta 3 adrenergic receptor agonist

Project description:Experiment designed to identify differences in gene expression profile in white adipose tissue upon stimulation by beta 3 adrenergic receptor agonist. This series compares 2 groups of C57BL/6J acutely treated with CL-316,243 or Saline for 3 hours.

2010-06-11 | E-GEOD-2674 | biostudies-arrayexpress

EDL933 in human macrophage THP-1 cells at different time point

Project description:Effect of human macrophages phagocytosis on the global transcriptome of EDL933 at different time. Infection of THP-1 with EDL933. Application of SCOTS technique to have cDNA. Hybridation on microarray. Reference: EDL933 in RPMI medium Keywords: stres response, host-bacteria response, time-course For each time point, 2 microarray are analysed and on each microarray there is 2 replicate Total of 4 replicate for each time point (including reference medium)

2010-06-24 | E-GEOD-9671 | biostudies-arrayexpress

Analysis of Rice microRNAs Expression under Drought Stress

Project description:We used this microarray to monitor rice miRNA expression profile under osmotic stress. Most of rice miRNAs have no significant change. miR-169f showed osmotic-responsive up-regulation. However, the others members of miR-169 family displays no responses to the osmotic stress. Keywords: microRNA,rice,osmotic stress,miR-169 The six selected time points after PEG treatment were 0h, 0.5h, 2h, 6h, 24h and 48h. For each sample, two independent small RNA libraries (biological replications) were generated and two independent reverse transcription/amplifications/hybridizations (technical replications) were performed for each library. Thus, for each sample data was collected from four independent hybridizations.

2010-06-11 | E-GEOD-5986 | biostudies-arrayexpress

Overexpression of the plant LAMMER kinase PK12

Project description:Comparison of plants that overexpress PK12 and non-transformed plants. Plants were grown for 10 days under long day conditions (GSM3816-GSM3819) or plants were grown for 21 days under long day conditions (GSM3820-GSM3823) . Hybridization was performed on arrays from Arabidopsis Keck Resource Lab Array (Yale University) containing 12K (GSM3816-GSM3819) or 9K (GSM3820-GSM3823)Arabidopsis ESTs. The 4 samples corresponding to each array represent 2 repetitions of 2 independent biological experiments. Keywords = Alternative splicing Keywords = phosphorylation

2003-02-04 | E-GEOD-274 | biostudies-arrayexpress

Agricultural sterol biosynthesis inhibitor fungicides

Project description:Resistance to agricultural fungicides in the field has created a need for discovering fungicides with new modes of action. DNA microarrays, because they provide information on expression of many genes simultaneously, could help to identify the modes of action. To begin an expression pattern database for agricultural fungicides, transcriptional patterns of Saccharomyces cerevisiae strain S288C genes were analysed following 2-h treatments with I50 concentrations of ergosterol biosynthesis inhibitors commonly used against plant pathogenic fungi. Eight fungicides, representing three classes of ergosterol biosynthesis inhibitors, were tested. To compare gene expression in response to a fungicide with a completely different mode of action, a putative methionine biosynthesis inhibitor (MBI) was also tested. Expression patterns of ergosterol biosynthetic genes supported the roles of Class I and Class II inhibitors in affecting ergosterol biosynthesis, confirmed that the putative MBI did not affect ergosterol biosynthesis, and strongly suggested that in yeast, the Class III inhibitor did not affect ergosterol biosynthesis. The MBI affected transcription of three genes involved in methionine metabolism, whereas there were essentially no effects of ergosterol synthesis inhibitors on methionine metabolism genes. There were no consistent patterns in other up- or downregulated genes between fungicides. These results suggest that inspection of gene response patterns within a given pathway may serve as a useful first step in identifying possible modes of action of fungicides. agricultural sterol biosynthesis inhibitor fungicides. Keywords = agriculture Keywords = ergosterol Keywords = methionine Keywords = fungicide Keywords = Saccharomyces cerevisiae S288C Keywords = biosynthesis

2010-06-10 | E-GEOD-2412 | biostudies-arrayexpress

Immune-stimulated Peyer's patch

Project description:RNA from all normal tissues were pooled in equal amounts and used to create the normal tissue library. RNA from all immune stimulated tissues (Salmonella infection, CT+LPS, and PBC, with and without cycloheximide) were pooled in equal amounts and used to create the immune stimulated tissue library. The subtracted library was created by combining the normal and immune stimulated libraries and by subtracting porcine fibroblast RNA.

2010-06-05 | E-GEOD-1970 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data