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Transcription profiling of Atlantic salmon cell line SHK-1 exposed to interferon type 1 and interferon gamma for 6 or 24 hours

ABSTRACT: We have stimulated an Atlantic salmon cell line, SHK-1, with both type I and type II recombinant salmonid IFNs and analysed the transcriptional response by microarray analysis. Cells were exposed to recombinant IFNs for 6 or 24 h or were left unexposed as controls. RNA was hybridised to a 17k feature TRAITS Atlantic salmon cDNA microarray in order to assess differential gene expression in response to IFN exposure.

ORGANISM(S): Salmo salar

SUBMITTER: Samuel Martin

PROVIDER: E-MEXP-1023 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Interferon type I and type II responses in an Atlantic salmon (Salmo salar) SHK-1 cell line by the salmon TRAITS/SGP microarray.

Martin S A M SA Taggart J B JB Seear P P Bron J E JE Talbot R R Teale A J AJ Sweeney G E GE Høyheim B B Houlihan D F DF Tocher D R DR Zou J J Secombes C J CJ

Physiological genomics 20070905 1

Interferons (IFNs) are cytokines that have proinflammatory, antiviral, and immunomodulatory effects and play a central role during a host response to pathogens. The IFN family contains both type I and type II molecules. While there are a number of type I IFNs, there is only one type II IFN. Recently both type I and type II IFN genes have been cloned in salmonid fish and recombinant proteins produced showing IFN activity. We have stimulated an Atlantic salmon cell line (SHK-1) with both type I an ...[more]

PMID: 17804604

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