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Transcription profiling of hypergonadal mice (Gnrh1 deletion) mouse testes vs. wild-type to examine the effects of the endogenous lack of gonadotropins on apoptosis

ABSTRACT: A comparison of testis transcriptional profile in hypogonadal (hpg) mice and age-matched wild-type controls

INSTRUMENT(S): Scanning hardware

ORGANISM(S): Mus musculus

SUBMITTER: Peter Ellis

PROVIDER: E-MEXP-1153 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Hypogonadal mouse, a model to study the effects of the endogenous lack of gonadotropins on apoptosis.

Chausiaux Oriane E OE Abel Margaret H MH Baxter Fiona O FO Khaled Walid T WT Ellis Peter J I PJ Charlton Harry M HM Affara Nabeel A NA

Biology of reproduction 20070801 1

Testicular apoptosis is involved in the regulation of germ cell numbers, allowing optimal sperm production. Apoptosis has been described to occur in response to the absence of hormonal stimulation of the testis. Here we investigate the effect of the physiological lack of gonadotropins from birth using the hypogonadal (homozygous for the mutant allele Gnrh1(hpg)) mouse as a model. We pursued a concerted strategy using microarray analysis and RT-PCR to assess transcript levels, TUNEL to quantify t ...[more]

PMID: 17671269

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