Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

61 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of human HeLa cells follwing siRNA mediated knock down of the arginine methyltransferases CARM1 and/or PRMT1

ABSTRACT: siRNA mediated knockdown of the arginine methyltransferases CARM1 and/or PRMT1 in HeLa cells

INSTRUMENT(S): ScanArray Express HT [PerkinElmer]

ORGANISM(S): Homo sapiens

SUBMITTER: Uta-Maria Bauer

PROVIDER: E-MEXP-1173 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

The protein arginine methyltransferases CARM1 and PRMT1 cooperate in gene regulation.

Kleinschmidt Markus A MA Streubel Gundula G Samans Birgit B Krause Michael M Bauer Uta-Maria UM

Nucleic acids research 20080415 10

Protein arginine methyltransferases (PRMT) have been implicated in the regulation of transcription. They are recruited to promoters via interaction with transcription factors and exert their coactivator function by methylating arginine residues in histones and other chromatin proteins. Here, we employ an unbiased approach to identify novel target genes, which are under the control of two members of the enzyme family, PRMT1 and CARM1/PRMT4 (coactivator associated arginine methyltransferase 1). By ...[more]

PMID: 18413343

Similar Datasets

Transcription profiling of human MALT lymphoma and chronic gastritis tissue from the same patients

Project description:21 Tissue samples of gastric MALT lymphoma were compared by cDNA microarray studies with chronic gastritis tissues from the same patient. From each patient, multiple mucosal biopsies of the stomach were taken both from i) the macroscopically infiltrated area and ii) distant from the lymphoma. For conventional histological examination, biopsies taken from the same areas were formalin fixed, embedded in paraffin and reviewed by a central pathologist. All lymphoma samples were tested by RT-PCR for t(11;18). None of them was t(11;18) positive.

2007-07-24 | E-MEXP-1168 | biostudies-arrayexpress

Transcription profiling of whole lymph node RNA from mice with different Suv39h1 genotypes

Project description:Genome-wide expression analysis was performed on RNA from whole neoplastic lymph nodes derived from individual Eµ-myc transgenic and Eµ-myc transgenic; Suv39h1-/- mice and from normal wildtype spleens as control.

2008-02-20 | E-MEXP-1423 | biostudies-arrayexpress

Transcription profiling of human Soas-2 cells infected with adenoviruses expressing p53 proteins with charge-neutralizing or charge-inverting mutations in the H1 helix to investigate the role of the dimerization interface in p53 function

Project description:To investigate the role of the dimerization interface for p53 function, we introduced modest charge-neutralizing (R181→L "EL") and more severe charge-inverting (R181→E "EE" and E180→R, R181→E "RE") mutations into the H1 helix of the full-length p53 molecule. To characterize the nuclear transcriptional function of p53 mutants in an unbiased manner we employed gene expression profiling using cDNA microarrays. Saos-2 cells were infected with adenoviruses expressing the p53 proteins EE, EL, WT and RE which span the entire spectrum of apoptotic activity. Total cellular RNA was isolated 18 hours after infection when apoptosis had not yet occurred.

2010-04-01 | E-MEXP-1209 | biostudies-arrayexpress

Transcription profiling of short-term cultured Myc transgenic vs. Myc transgenic Suv39h1 deficient mouse lymphoma cells with and without exposure to 100 pM of human TGF-b1

Project description:Genome-wide expression analysis was performed on RNA from short-term cultured Eµ-myc transgenic vs. Eµ-myc transgenic; Suv39h1-/- lymphoma cells with and without exposure to 100 pM of human TGF-b1 for 24 hours, with wildtype B-cells as control

2008-02-20 | E-MEXP-1424 | biostudies-arrayexpress

Transcription profiling time series of mouse melanocytes transfected with an inducible version of the oncogenic receptor Xmrk, starved for 72 h and afterwards stimulated with EGF

Project description:Signaling pathways specifically induced by the oncogenic receptor Xmrk can be elucidated by comparing gene expression in cells with the activated receptor to gene expression in cells with the non-activated receptor. Mouse melanocytes transfected with an inducible version of the oncogenic receptor Xmrk, were starved for 72 h and afterwards stimulated with EGF. We compared cells stimulated for 15 minutes, 1 h, 2 h, 4 h, 8 h or 24 h, respectively, to unstimulated cells.

2008-08-31 | E-MEXP-1311 | biostudies-arrayexpress

Transcription profiling of mouseB-cell lymphomas with defined genetic alterations in p53 or p73

Project description:Induction of apoptosis by the tumor suppressor p53 is known to protect from Myc-driven lymphomagenesis. The p53 family member p73 is also a pro-apoptotic protein, which is activated in response to oncogenes like Myc. We therefore investigated whether p73 provides a similar protection from Myc-driven lymphomas as p53. To generate B-cell lymphomas with defined genetic alterations in p53 or p73, we crossed the Eµ-Myc transgenic to mice heterozygous for germ-line deletions in p53 (p53+/) or p73 (p73+/-). Lymphomas which have spontaneously developed in Eµ-Myc transgenic animals with the genotypes p53+/+, p53+/-, p73+/+, p73+/- or p73-/- were isolated when the animals were moribund and further processed for gene expression profiling with 22.5K cDNA microarrays.

2008-12-05 | E-MEXP-1620 | biostudies-arrayexpress

RNAi knock down of AURKA in human neuroblastoma IMR-32 cells

Project description:IMR-32 cells were infected either with control-shRNA or one of two different AURKA-shRNA vectors to identify genes regulated after AURKA knockdown.

2008-06-01 | E-MEXP-1496 | biostudies-arrayexpress

siRNAi knockdown of ZBTB4 in human SH-EP cells reveals Zbtb4 represses transcription of P21CIP1 and controls the cellular response to p53 activation

Project description:SH-EP cells were depleted for ZBTB4; Transfection of the cells were performed by oligofectamin RNAimax (Invitrogen) with siRNA smart pool (Dharmacon RNA Technologies); total RNA was isolated using RNAeasy Mini Kit (QIAGEN), preamplified, labeled with Cy3 and Cy5, respectively, and hybridized to cDNA-microarrays

2008-07-01 | E-MEXP-1554 | biostudies-arrayexpress

Transcription profiling of human Wilms tumor samples to select genes responsible for tumor development, metastasis and progression

Project description:Expression analysis was performed in 77 Wilms tumors in order to select genes responsible for tumor development, metastasis and progression.

2009-02-05 | E-MEXP-221 | biostudies-arrayexpress

Response of a resistant pea accesion to Mycosphaerella pinodes

Project description:Plants of the resistant Pisum sativum subsp. syriacum accession P665 and the susceptible pea cultivar Messire were inoculated with M. pinodes.The experiment was conducted in three replicates. 16, 24 and 48 hours after inoculation RNA was isolated from leaves of infected plants and transcribed into cDNA. For each time point and replicate, Cy-labelled cDNA samples from resistant and susceptible plants were mixed and hybridized to Mt16kOLI1Plus microarray

2011-08-24 | E-TABM-1084 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data