Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of three Bordetella bronchiseptica strains at three distinct phases of growth: mid-log phase, late-log phase, and late-stationary phase

ABSTRACT: Microarray analysis was performed to determine if the growth-dependent gene expression changes occurred on a global scale for B. bronchiseptica strains RB50, RB53, and RB54. For this analysis cDNA representing three distinct phases of growth: mid-log phase (15H), late-log phase (24H), and late-stationary phase (48H) were directly compared.

ORGANISM(S): Bordetella bronchiseptica

SUBMITTER: Tracy Nicholson

PROVIDER: E-MEXP-2015 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Microarray and functional analysis of growth phase-dependent gene regulation in Bordetella bronchiseptica.

Nicholson Tracy L TL Buboltz Anne M AM Harvill Eric T ET Brockmeier Susan L SL

Infection and immunity 20090810 10

Growth phase-dependent gene regulation has recently been demonstrated to occur in Bordetella pertussis, with many transcripts, including known virulence factors, significantly decreasing during the transition from logarithmic to stationary-phase growth. Given that B. pertussis is thought to have derived from a Bordetella bronchiseptica-like ancestor, we hypothesized that growth phase-dependent gene regulation would also occur in B. bronchiseptica. Microarray analysis revealed and quantitative re ...[more]

PMID: 19667046

Similar Datasets

Transcription profiling of Bordetella bronchiseptica strains RM50 and 1289

Project description:B. bronchiseptica strains RB50 and 1289 were grown in SS broth, subcultured at a starting OD600 of 0.02 into 50mL of SS broth, grown at 37C for 24 hours while shaking and harvested in log phase (OD600 1.0). Total RNA was extracted with Trizol (Invitrogen, Carlsbad, CA), treated with RNase-free DNase I (Invitrogen, Carlsbad, CA) and purified using RNeasy columns (Qiagen, Valencia, CA) according to the manufacturers instructions. RNA was isolated from two independent biological replicates of strains RB50 and 1289. A 2-color hybridization format was used and dye-swap experiments were performed. For each reaction, 5ug of cDNA was fluorescently labeled. The two differentially labeled reactions to be compared were combined and hybridized to a B. bronchiseptica strain RB50 specific long-oligonucleotide microarray.

2009-01-01 | E-MEXP-1736 | biostudies-arrayexpress

Overlap of RisA and RisX regulons

Project description:To investigate the role of RisX in regulating the RisA regulon, we designed and assembled a long-oligonucleotide microarray representing the full B. pertussis genome. For these experiments RNA was extracted from wild-type B. pertussis strain Bp536, ?risA, and ?risX isogenic mutants, converted to cDNA, labeled fluorescently labeled and directly compared by hybridizion to a B. pertussis long-oligonucleotide microarray.

2012-11-01 | E-MEXP-3728 | biostudies-arrayexpress

Transcription profiling of Bordetella bronchiseptica virulent swine isolate KM22, wild type and fhaB and prn deletion mutants

Project description:B. bronchiseptica strain KM22, a virulent swine isolate, and strains TN27 and TN28, fhaB and prn deletion mutants of KM22, respectively, were cultured in Stainer-Scholte broth and grown at 37C with shaking at 275 rpm until an OD600 of 0.8 was reached. RNA extracted from KM22 was then compared to RNA extracted from TN27 and TN28.

2008-09-20 | E-MEXP-1558 | biostudies-arrayexpress

Transcription profiling of Bordetella bronchiseptica strains 253 and RB50 to investigate adenylate cyclase toxin (ACT) expression

Project description:Bordetella bronchiseptica is a gram-negative respiratory pathogen that causes a diverse spectrum of respiratory disease in a wide-range of hosts. We sought to determine if strains of B. bronchiseptica differed in virulence using the mouse model of infection. Mean lethal doses (LD50) of different B. bronchiseptica strains varied widely in the murine model. B. bronchiseptica strain 253 had a LD50 that was 10-fold lower than the prototypical and fully sequenced B. bronchiseptica strain RB50. Using whole genomic transcriptome analysis covering 100% of B. bronchisetpctica strain RB50ÃÂÃÂs predicted open reading frames (ORFs), 253 was identified as lacking expression of adenylate cyclase toxin (ACT).

2007-10-01 | E-MEXP-1203 | biostudies-arrayexpress

Transcription profiling of Bordetella bronchiseptica RB50 and its Bvg- phase-locked derivative, RB54 to test and validate a long oligonucleotide B. bronchiseptica microarray

Project description:Based on the genomic sequence and using the freely available software ArrayOligoSelector, a long oligonucleotide B. bronchiseptica microarray was designed and assembled. This long-oligonucleotide microarray was subsequently tested and validated by comparing changes in the global expression profiles between B. bronchiseptica RB50 and its Bvg- phase-locked derivative, RB54.

2007-01-31 | E-MEXP-961 | biostudies-arrayexpress

BpsR is a dual and essential transcriptional regulator and attenuates respiratory tract colonization of Bordetella

Project description:To identify the regulated by the transcriptional regulator BpsR, whole genome transcriptome analysis performed to determine which genes were differentially expressed between an in-frame bpsR deletion-mutant of B. bronchiseptica and its wild-type parental strain RB50.

2016-06-30 | E-MTAB-2881 | biostudies-arrayexpress

Transcription profiling by array of Bordetella bronchiseptica strains RB50 and 761 grown in normal or high carbon dioxide conditions

Project description:Comparison of B. bronchiseptica strains RB50 and 761 grown in either atmospheric concentrations of oxygen and carbon dioxide (normal conditions) or in atmospheric levels of oxygen with the addition of 5% carbon dioxide into a sealed incubator (5% CO2 conditions).

2010-10-01 | E-MEXP-2875 | biostudies-arrayexpress

Biofilm development in B. bronchiseptica

Project description:To identify global stage-specific gene sets unique to biofilm formation for B. bronchiseptica, whole-genome transcriptome analysis was used to measure mRNA abundance under biofilm and planktonic growth conditions after 6, 12, 24, 36, and 48 hours of growth.

2012-01-01 | E-MEXP-3295 | biostudies-arrayexpress

T44625

Project description:Whole genome transcriptome analysis was performed to determine which genes were differentially expressed between strains T44625 and RB50

2013-12-01 | E-MEXP-3967 | biostudies-arrayexpress

T44625-cgh

Project description:comparative genomic hybridization (CGH) analysis was perfromed in order to identify coding regions present in strain T44625 that are either absent or contain a high degree of sequence divergence compared to the RB50 reference strain.

2013-12-01 | E-MEXP-3968 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data