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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNAi knock down of the DNA-repair enzyme and transcriptional co-activator APE1 in human HeLa cells

ABSTRACT: Transcriptional profiling was done following APE1/Ref1 silencing using conditional gene expression knockdown by RNA interference (RNAi) technology in HeLa cell lines. APE1 is a DNA-repair enzyme and transcriptional co-activator. Knockdown leads to cell-growth arrest, apoptosis and impairment in the intracellular redox state and cytoskeletal perturbation.

ORGANISM(S): Homo sapiens

SUBMITTER: Leo Zeef

PROVIDER: E-MEXP-1315 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide analysis and proteomic studies reveal APE1/Ref-1 multifunctional role in mammalian cells.

Vascotto Carlo C Cesaratto Laura L Zeef Leo A H LA Deganuto Marta M D'Ambrosio Chiara C Scaloni Andrea A Romanello Milena M Damante Giuseppe G Taglialatela Giulio G Delneri Daniela D Kelley Mark R MR Mitra Sankar S Quadrifoglio Franco F Tell Gianluca G

Proteomics 20090201 4

Apurinic apyrimidinic endonuclease/redox effector factor 1 (APE1/Ref-1) protects cells from oxidative stress by acting as a central enzyme in base excision repair pathways of DNA lesions and through its independent activity as a redox transcriptional co-activator. Dysregulation of this protein has been associated with cancer development. At present, contrasting data have been published regarding the biological relevance of the two functions as well as the molecular mechanisms involved. Here, we ...[more]

PMID: 19180539

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