Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Mycobacterium tuberculosis H37Rv and the isogenic mprA::Kmr mutant

ABSTRACT: Whole genome expression analyses between wild-type Mycobacterium tuberculosis H37Rv and the isogenic mprA::Kmr mutant. Expression analyses between these strains were conducted following growth under physiological conditions, or following exposure to non-inhibitory concentrations of SDS or Triton X-100.

INSTRUMENT(S): ScanArray 5000 [PerkinElmer]

ORGANISM(S): Mycobacterium tuberculosis

SUBMITTER: Thomas Zahrt

PROVIDER: E-MEXP-552 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

MprAB is a stress-responsive two-component system that directly regulates expression of sigma factors SigB and SigE in Mycobacterium tuberculosis.

He Hongjun H Hovey Raymond R Kane Jason J Singh Vineet V Zahrt Thomas C TC

Journal of bacteriology 20060301 6

The genetic mechanisms mediating the adaptation of Mycobacterium tuberculosis within the host are poorly understood. The best-characterized regulatory systems in this organism include sigma factors and two-component signal transduction systems. mprAB is a two-component system required by M. tuberculosis for growth in vivo during the persistent stage of infection. In this report, we demonstrate that MprAB is stress responsive and regulates the expression of numerous stress-responsive genes in M. ...[more]

PMID: 16513743

Similar Datasets

Transcription profiling of mouse wild type adult male mouse livers vs transgenic mice expressing human Small Heterodimer Partner in hepatocytes

Project description:Comparison of gene expression in adult male mouse livers between wildtype mice and Transgenic mice expressing human Small Heterodimer Partner in hepatocytes.

2006-01-01 | E-MEXP-294 | biostudies-arrayexpress

Transcription profiling of blasts from three APL patients expressing PML/RAR before and after treatment with 1 uM retinoic acid (RA) in vitro for four hours

Project description:Gene expression profiles in blasts from three APL patients expressing PML/RAR were assessed before and after treatment with 1 uM retinoic acid (RA) in vitro for four hours. We then studied a U937 clone conditionally expressing PML/RAR (U937-PR), (Grignani et al. 1993) (Alcalay et al. 2003), and compared the gene expression profile prior to and after 4 hours of treatment with 1 uM RA, to that obtained from a cell line bearing an empty vector (U937-MT). For each sample, biotinylated cRNA targets were synthesized starting from 5ug of total RNA, and hybridized to the complete set of HG-U133 Affymetrix oligonucleotide chips, which explores the expression of approximately 45,000 human transcripts. Results were analyzed using MASv5 and further elaborated with the GenePicker software. GeneChip probe sets regulated by RA in each sample were clustered into non-redundant regulated genes according to UniGene release Hs.166.

2004-12-31 | E-MEXP-149 | biostudies-arrayexpress

Transcription profiling of dissected neuroepithelium (Neur.) and from all other non-neuroepithelial tissues (N. Neur for Non Neuronal tissues) from six wild type (WT) and six Mdm4-null mouse embryos (KO)

Project description:Total RNA was isolated from the dissected neuroepithelium (Neur.) and from all other non-neuroepithelial tissues (N. Neur for Non Neuronal tissues) from six wild-type (WT) and six Mdm4-null embryos (KO). Equal quantities of total RNA from single samples were used to obtain four RNA pools (WT-Neur, WT-N.Neur, KO-Neur, KO-N.Neur), each representing one of the experimental samples under analysis. Biotin labeled cRNA targets were obtained starting from 10ug of total RNA derived from the RNA pools. cDNA synthesis was performed with GIBCO SuperScript Custom cDNA Synthesis Kit, and biotin-labeled antisense RNA was transcribed in vitro using Ambion's In Vitro Transcription System, and including Bio-16-UTP and Bio-11-CTP (Enzo) in the reaction. Each labeled target was hybridized to two copies of the the complete Affymetrix MG-U174v2 chip set.

2004-12-31 | E-MEXP-155 | biostudies-arrayexpress

Transcription profiling of 4 week old BALB/c_Fech m1Pas mice (Fech) vs with 4 week old control BALB/c mice

Project description:Comparison of liver gene expression of 4 week old BALB/c_Fech m1Pas mice(Fech mice for short) with 4 week old control BALB/c mice.

2005-10-21 | E-MEXP-194 | biostudies-arrayexpress

Transcription profiling of human fibroblast RNA from patient with a mitochondrial oxidative phosphorylation complex I deficency vs normal fibroblast RNA

Project description:Comparison of fibroblast RNA from patient with a mitochondrial oxidative phosphorylation complex I deficency to normal fibroblast RNA.<br><br>Note: this experiment submission is not fully MIAME compliant, due to the unavailability of oligonucleotide sequences on the A-MEXP-70 array ("Adelaide, H.sapiens, 19k array, version 1").

2004-09-01 | E-MEXP-118 | biostudies-arrayexpress

Transcription profiling of human fibroblast RNA from a patient with a mitochondrial oxidative phosphorylation complex I deficiency vs normal fibroblast RNA

Project description:Comparison of fibroblast RNA from a patient with a mitochondrial oxidative phosphorylation comnplex I deficiency against normal fibroblast RNA.<br><br>Note: this experiment submission is not fully MIAME compliant, due to the unavailability of oligonucleotide sequences on the A-MEXP-70 array ("Adelaide, H.sapiens, 19k array, version 1").

2004-09-01 | E-MEXP-119 | biostudies-arrayexpress

Metadata Entered in Submitting Proteomic Data to PRIDE

Project description:For the identification of potential pathways and their interactions that underlie a complex change in cellular state, such as neurite outgrowth, we stimulated the neuroblastoma cell line N2A with HU210, a potent activator of the cannabinoid receptor type I, followed by transcriptomic and proteomic analysis. Differentially expressed genes and proteins obtained at multiple timepoints were subjected to standard and dynamic enrichment analysis using the Molecular Biology of the Cell Ontology. Many of the identified pathways described constitutive cellular functions. Involvement of predicted pathways in neurite outgrowth was confirmed by siRNA knock-down of pathway representative genes in primary rat cortical neurons. Dynamic modeling of pathway activities suggested that the identified increase in vesicle membrane back-transported from the growth cone to the cell body ensures recycling of components needed by forward moving vesicle.

2022-10-14 | PXD036706 | Pride

Transcription profiling of brains from anarchist and wild-type honeybee strains to investigate ovary activation

Project description:Effect of genotype (wildtype vs. anarchist) on gene expression in developing (four-day-old) honeybee worker brains. Goal of experiment is to identify genes associated with ovary activation during worker development. These genes are hypothesised to be candidates for the regulation of worker sterility.

2006-10-01 | E-MEXP-699 | biostudies-arrayexpress

Transcription profiling of abdomens from anarchist and wild-type honeybee strains to investigate ovary activation

Project description:Effect of genotype (wildtype vs. anarchist) on gene expression in developing (four-day-old) honeybee worker abdomens. Goal of experiment is to identify genes associated with ovary activation during worker development. These genes are hypothesised to be candidates for the regulation of worker sterility.

2006-10-01 | E-MEXP-705 | biostudies-arrayexpress

Transcription profiling of synchronized Sulfolobus acidocaldarius populations as they progress through the cell cycle

Project description:Exponentially growing Sulfolobus acidocaldarius were treated with NaAc to generate replication runout and arrest in G2 phase. The cells were then resuspended in fresh acetate-free media which generates a synchronous population. Samples for investigation of gene expression change were taken during the synchronised populations progress through the cell cycle.

2007-12-08 | E-MEXP-936 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data