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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of brains from anarchist and wild-type honeybee strains to investigate ovary activation

ABSTRACT: Effect of genotype (wildtype vs. anarchist) on gene expression in developing (four-day-old) honeybee worker brains. Goal of experiment is to identify genes associated with ovary activation during worker development. These genes are hypothesised to be candidates for the regulation of worker sterility.

INSTRUMENT(S): 428 [Affymetrix]

ORGANISM(S): Apis mellifera

SUBMITTER: Benjamin Oldroyd

PROVIDER: E-MEXP-699 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Towards a molecular definition of worker sterility: differential gene expression and reproductive plasticity in honey bees.

Thompson G J GJ Kucharski R R Maleszka R R Oldroyd B P BP

Insect molecular biology 20061001 5

We show that differences in the reproductive development of honey bee workers are associated with locus-specific changes to abundance of messenger RNA. Using a cross-fostering field experiment to control for differences related to age and environment, we compared the gene expression profiles of functionally sterile workers (wild-type) and those from a mutant strain in which workers are reproductively active (anarchist). Among the set of three genes that are significantly differentially expressed ...[more]

PMID: 17069629

Similar Datasets

Transcription profiling of abdomens from anarchist and wild-type honeybee strains to investigate ovary activation

Project description:Effect of genotype (wildtype vs. anarchist) on gene expression in developing (four-day-old) honeybee worker abdomens. Goal of experiment is to identify genes associated with ovary activation during worker development. These genes are hypothesised to be candidates for the regulation of worker sterility.

2006-10-01 | E-MEXP-705 | biostudies-arrayexpress

Metadata Entered in Submitting Proteomic Data to PRIDE

Project description:For the identification of potential pathways and their interactions that underlie a complex change in cellular state, such as neurite outgrowth, we stimulated the neuroblastoma cell line N2A with HU210, a potent activator of the cannabinoid receptor type I, followed by transcriptomic and proteomic analysis. Differentially expressed genes and proteins obtained at multiple timepoints were subjected to standard and dynamic enrichment analysis using the Molecular Biology of the Cell Ontology. Many of the identified pathways described constitutive cellular functions. Involvement of predicted pathways in neurite outgrowth was confirmed by siRNA knock-down of pathway representative genes in primary rat cortical neurons. Dynamic modeling of pathway activities suggested that the identified increase in vesicle membrane back-transported from the growth cone to the cell body ensures recycling of components needed by forward moving vesicle.

2022-10-14 | PXD036706 | Pride

Transcription profiling of mouse wild type adult male mouse livers vs transgenic mice expressing human Small Heterodimer Partner in hepatocytes

Project description:Comparison of gene expression in adult male mouse livers between wildtype mice and Transgenic mice expressing human Small Heterodimer Partner in hepatocytes.

2006-01-01 | E-MEXP-294 | biostudies-arrayexpress

Transcription profiling of dissected neuroepithelium (Neur.) and from all other non-neuroepithelial tissues (N. Neur for Non Neuronal tissues) from six wild type (WT) and six Mdm4-null mouse embryos (KO)

Project description:Total RNA was isolated from the dissected neuroepithelium (Neur.) and from all other non-neuroepithelial tissues (N. Neur for Non Neuronal tissues) from six wild-type (WT) and six Mdm4-null embryos (KO). Equal quantities of total RNA from single samples were used to obtain four RNA pools (WT-Neur, WT-N.Neur, KO-Neur, KO-N.Neur), each representing one of the experimental samples under analysis. Biotin labeled cRNA targets were obtained starting from 10ug of total RNA derived from the RNA pools. cDNA synthesis was performed with GIBCO SuperScript Custom cDNA Synthesis Kit, and biotin-labeled antisense RNA was transcribed in vitro using Ambion's In Vitro Transcription System, and including Bio-16-UTP and Bio-11-CTP (Enzo) in the reaction. Each labeled target was hybridized to two copies of the the complete Affymetrix MG-U174v2 chip set.

2004-12-31 | E-MEXP-155 | biostudies-arrayexpress

Transcription profiling of blasts from three APL patients expressing PML/RAR before and after treatment with 1 uM retinoic acid (RA) in vitro for four hours

Project description:Gene expression profiles in blasts from three APL patients expressing PML/RAR were assessed before and after treatment with 1 uM retinoic acid (RA) in vitro for four hours. We then studied a U937 clone conditionally expressing PML/RAR (U937-PR), (Grignani et al. 1993) (Alcalay et al. 2003), and compared the gene expression profile prior to and after 4 hours of treatment with 1 uM RA, to that obtained from a cell line bearing an empty vector (U937-MT). For each sample, biotinylated cRNA targets were synthesized starting from 5ug of total RNA, and hybridized to the complete set of HG-U133 Affymetrix oligonucleotide chips, which explores the expression of approximately 45,000 human transcripts. Results were analyzed using MASv5 and further elaborated with the GenePicker software. GeneChip probe sets regulated by RA in each sample were clustered into non-redundant regulated genes according to UniGene release Hs.166.

2004-12-31 | E-MEXP-149 | biostudies-arrayexpress

Transcription profiling of abdomens from wild type and anarchistic strains of worker honey bees to identify genes related to ovary activation

Project description:This experiment examines differences in gene expression between wildtype and an experimental strain (anarchistic) of worker honey bees (Apis mellifera). Mature wildtype and anarchistic workers tend to have non-active and active ovaries, respectively. Thus young workers from these strains are expected to show differential expression at loci involved in the regulation of worker reproduction, which occurs via arrhenotokous parthenogenesis.

2008-12-09 | E-MEXP-1549 | biostudies-arrayexpress

Transcription profiling of genes related to ovary activation in worker honey bee brains

2008-07-11 | E-MEXP-1552 | biostudies-arrayexpress

Transcription profiling of Mycobacterium tuberculosis H37Rv and the isogenic mprA::Kmr mutant

Project description:Whole genome expression analyses between wild-type Mycobacterium tuberculosis H37Rv and the isogenic mprA::Kmr mutant. Expression analyses between these strains were conducted following growth under physiological conditions, or following exposure to non-inhibitory concentrations of SDS or Triton X-100.

2006-03-01 | E-MEXP-552 | biostudies-arrayexpress

Transcription profiling of 4 week old BALB/c_Fech m1Pas mice (Fech) vs with 4 week old control BALB/c mice

Project description:Comparison of liver gene expression of 4 week old BALB/c_Fech m1Pas mice(Fech mice for short) with 4 week old control BALB/c mice.

2005-10-21 | E-MEXP-194 | biostudies-arrayexpress

Transcription profiling of human fibroblast RNA from patient with a mitochondrial oxidative phosphorylation complex I deficency vs normal fibroblast RNA

Project description:Comparison of fibroblast RNA from patient with a mitochondrial oxidative phosphorylation complex I deficency to normal fibroblast RNA.<br><br>Note: this experiment submission is not fully MIAME compliant, due to the unavailability of oligonucleotide sequences on the A-MEXP-70 array ("Adelaide, H.sapiens, 19k array, version 1").

2004-09-01 | E-MEXP-118 | biostudies-arrayexpress

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