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Chromatin immunoprecipitation to determine p53 binding in several human cell types

ABSTRACT: binding of p53 in peripherial blood mononuclears

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Homo sapiens

SUBMITTER: Idit Shiff

PROVIDER: E-MEXP-1501 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chromatin immunoprecipitation-on-chip reveals stress-dependent p53 occupancy in primary normal cells but not in established cell lines.

Shaked Helena H Shiff Idit I Kott-Gutkowski Miriam M Siegfried Zahava Z Haupt Ygal Y Simon Itamar I

Cancer research 20081201 23

The p53 tumor suppressor protein is a transcription factor that plays a key role in the cellular response to stress and cancer prevention. Upon activation, p53 regulates a large variety of genes causing cell cycle arrest, apoptosis, or senescence. We have developed a p53-focused array, which allows us to investigate, simultaneously, p53 interactions with most of its known target sequences using the chromatin immunoprecipitation (ChIP)-on-chip methodology. Applying this technique to multiple cell ...[more]

PMID: 19047144

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