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Chromatin immunoprecipitation of mouse and human cell lines and tissues using E2F4 antibodies

ABSTRACT: Despite being expressed in most tissues, a number of mammalian process-specific transcriptional regulators, such as those involved in cell cycle and immune responses, often have profound tissue-specific phenotypes. The mechanism underlying this effect is poorly understood. We chose to investigate on a genome-wide basis how the cell cycle master regulator E2F4, a key regulator of proliferation and differentiation in G0 cells, controls gene expression in multiple mammalian tissues. We identified potential direct targets of E2F4 in primary mouse and human tissues using chromatin immunoprecipitation. E2F4 binds a core set of genes in most mouse tissues that includes a substantial number of previously identified direct targets, and these common targets are highly enriched in the canonical binding sequence. Comparison of the genes bound in mouse tissues versus those bound in comparable human tissues revealed that, consistent with previous results, a core set of targets was bound in both species, and contains a substantial overrepresentation of cell-cycle genes.

INSTRUMENT(S): Axon GenePix 4000B scanning hardware

ORGANISM(S): Mus musculus

SUBMITTER: Duncan Odom

PROVIDER: E-TABM-272 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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