Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of rat mixed-tissue RNA samples in different laboratories to monitor proficiency

ABSTRACT: A pilot program for monitoring proficiency in microarray facilities using three replicates of two different RNA sources. Participating laboratories prepared and hybridized targets from the six RNA samples using their own protocols. The entire process was repeated three times over a nine-month period and included approximately fifteen distinct laboratories in each testing round.

ORGANISM(S): Rattus norvegicus

SUBMITTER: P Scott Pine 

PROVIDER: E-MEXP-1568 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Use of diagnostic accuracy as a metric for evaluating laboratory proficiency with microarray assays using mixed-tissue RNA reference samples.

Pine P S PS   Boedigheimer M M   Rosenzweig B A BA   Turpaz Y Y   He Y D YD   Delenstarr G G   Ganter B B   Jarnagin K K   Jones W D WD   Reid L H LH   Thompson K L KL  

Pharmacogenomics 20081101 11

Effective use of microarray technology in clinical and regulatory settings is contingent on the adoption of standard methods for assessing performance. The MicroArray Quality Control project evaluated the repeatability and comparability of microarray data on the major commercial platforms and laid the groundwork for the application of microarray technology to regulatory assessments. However, methods for assessing performance that are commonly applied to diagnostic assays used in laboratory medic  ...[more]

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