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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of pig granulosa cells after treatment with follicle-stimulating hormone to find genes potentially important in oocyte maturation and development

ABSTRACT: The FSH microarray was performed to identify new genes to be regulated by FSH in granulosa cells, in order to ultimately find genes potentially important in oocyte maturation and development.

ORGANISM(S): Sus scrofa

SUBMITTER: Carla Zijlstra

PROVIDER: E-MEXP-1763 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of pig ovary granulosa cells and granulosa cell and cumulus oocyte complexes

Project description:Ovaries from cyclic sows were derived from a local slaughterhouse and transported to the laboratory at 27-33ºC within 2 hours after death. After removal of surrounding tissue, the ovaries were rinsed with water (27-33ºC) and submersed in PBS containing 100U/ml penicillin and 100µg/ml streptomycin (Gibco, Breda, The Netherlands) at 37ºC. Granulosa cells and oocytes were aspirated with a 26G needle from healthy, 3-6 mm follicles. Follicular health was determined according to previously described criteria (28). Harvested cells were pooled and the cell suspension was washed twice by centrifugation at room temperature in McCoys 5a medium with glutamine (Invitrogen, Breda, The Netherlands) supplemented with 100U/ml penicillin, 100µg/ml streptomycin, 0.1% BSA (Fluka, Zwijndrecht, The Netherlands), 2.5µg/ml transferrin and 4ng/ml sodium selenite. Oocytes were removed from the cell suspension by filtration through a 70µm cell sieve (Becton en Dickinson, Alphen a/d Rijn, The Netherlands). The number of viable GCs was estimated by trypan blue exclusion. Granulosa cells were cultured in serum free medium as previously described by Picton and colleagues (26). For co-culture experiments cumulus oocyte complexes (COC) were aspirated from 3-6mm follicles. Cell debris and COC were allowed to settle at the bottom of the tube and the supernatant was discarded. Cumulus oocyte complexes were washed in TL-HEPES and subsequently selected by morphological appearance (dark cytoplasm and at least 2 layers of cumulus cells). The COC were transferred to wash medium containing 0.1U/l FSH, 100mg/l long R3-IGF-1, 10mg/l insulin (all from Sigma Aldrich, Zwijndrecht, The Netherlands) and 100mg/l testosterone (Fluka, Zwijndrecht, The Netherlands). Granulosa cells, 7.5*106 per well of a 6 wells plate, and COC, 80-100, were co-cultured in 3.5ml medium per well of a 6 wells plate. Cells were cultured in a humidified atmosphere at 37ºC and 5% CO2 for 48 hrs.

2010-03-31 | E-MEXP-1768 | biostudies-arrayexpress

Evaluation of methylmercury on neural development in Embryonic Stem Cell Differentiation Cultures

Project description:Effects of methylmercury on stem cell neural development at four differernt culture time points

2011-05-28 | E-TABM-1108 | biostudies-arrayexpress

Transcription profiling by array of human HepG2 cells treated with TCDD

Project description:The integration of different M-^SomicsM-^T technologies has already been shown in several in vivo studies to offer a complementary insight into cellular responses of toxic processes. We therefore hypothesize that the combining of transcriptomics and metabonomics data may improve the understanding of molecular mechanisms underlying non-genotoxic carcinogenicity in vitro. To test this hypothesis, the human hepatocarcinoma cell line HepG2 was exposed to the well known environmental pollutant TCDD. <br><br>A custom CDF file is available on the FTP site for this experiment (in file E-MEXP-2817.additional.zip) for use with the normalized data file for this experiment.

2011-07-01 | E-MEXP-2817 | biostudies-arrayexpress

Neural embryonic stem cell (ESTn) test dose-responses of Valproic Acid, Cyproconazole and Hexaconazole in the neural embryonic stem cell test.

Project description:Effects of Valproic Acid, Cyproconazole and Hexaconazole on stem cell neural development.

2012-07-17 | E-TABM-1205 | biostudies-arrayexpress

Transcription profiling by array of human B cell lymphoma cell lines in response to rituximab treatment

Project description:HF-1, Granta-519, SUDHL4,Oci-Ly10 and Oci-Ly3 cell lines, representing different B cell lymphoma subtypes, were induced with rituximab (R) for three hours and analyzed for differentially expressed genes compared to untreated control.

2010-06-22 | E-MEXP-2317 | biostudies-arrayexpress

Identification and relative quantification of hFSH glycoforms in women sera by PRM MS based approach.

Project description:This study presents an alternative preliminary strategy to identify and quantify serum hFSH glycoforms based on immunopurification assay and mass spectrometry (MS) using parallel reaction monitoring (PRM) analysis. In this study, we provide MS-PRM data acquisition method for hFSH glycopeptides identification with high specificity and their quantification by extracting the chromatographic traces of selected fragments of glycopeptides. Once set up in all its features, the proposed method could be transferred to the clinic to improve the fertility treatments and follow-ups in men and women.

2021-06-15 | PXD025585 | Pride

Transcription profiling of human hepatoma cell lines HepG2 and HepaRG using whole genome gene expression analysis before and after exposure to the GTX carcinogens AFB1 and BaP, and the NGTX carcinogens CsA, E2 and TCDD for 12 and 48 h

Project description:Direct comparison of the hepatoma cell lines HepG2 and HepaRG using whole genome gene expression analysis before and after exposure to the GTX carcinogens AFB1 and BaP and the NGTX carcinogens CsA, E2 and TCDD for 12 and 48 h.

2010-04-15 | E-MEXP-2458 | biostudies-arrayexpress

Transcription profiling of human duodenal mucosa samples taken from subjects after ingesting a Lactobacillus plantarum WCFS1 supplement for two weeks

Project description:The effects of a 2-week suppletion period with L. plantarum WCFS1 on duodenal gene expression profiles was investigated in healthy subjects in a placebo-controlled doouble-blind study. Each subject ingested a supplement containing L. Plantarum WCFS1 once a day. After that period, tissue samples from the horizontal part of the duodenum were obtained by standard flexible gastroduodenoscopy, and Affymetrix microarrays were used to determine genome-wide gene expression profiles.

2008-11-14 | E-MEXP-1336 | biostudies-arrayexpress

Transcription profiling by array of human intestinal mucosa after exposure to glutamine solution

Project description:Glutamine mediates different aspects of gut physiology. Its effects on gene expression is, however, not known. To examine the effect of glutamine on intestinal gene expression in vivo in healthy subjects, an intestinal catheter was inserted into the proximal part of the small intestine, to enable injection of a glutamine-containing solution. Glutamine injection occurred over a 4-h period. After this period, mucosal tissue samples from the horizontal part of the duodenum were obtained by standard flexible gastroduodenoscopy, and Affymetrix microarrays were used to determine genome-wide gene expression profiles.

2008-11-13 | E-MEXP-1335 | biostudies-arrayexpress

Transcription profiling by array of human duodenal mucosa from healthy subjects after treatment with the non-steroidal anti-inflammatory drug idomethacin or a placebo

Project description:Indomethacin is a non-steroidal anti-inflammatory drug. It is widely used in clinical practice. In scientific intervention studies, it is applied as a methodology to inflict reversible damage to gastrointestinal epithelium. The exact pathogenesis is not well understood. The present study aimed to obtain a better understanding of indomathacin-induced pathogenesis by determining its effects on gene expression in duodenal mucosa in healthy subjects. Tissue samples from the horizontal part of the duodenum were obtained by standard flexible gastroduodenoscopy, and Affymetrix microarrays were used to determine genome-wide gene expression profiles.

2008-11-15 | E-MEXP-1337 | biostudies-arrayexpress

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