Project description:Nucleosome mapping and histone H3 lysine 14 acetylation chromatin immuno-precipitation in two unrelated strains of yeast (BY and RM).
Project description:Quantitative variation of epigenetic marks, such as histone modifications, can modify gene expression and eventually contribute to inter-individual phenotypic variation. Our goal is to investigate natural inter-individual variation of the epigenome in a quantitative manner. To probe the degree of natural epigenomic diversity in S. cerevisiae, we compared two unrelated wild strains using replicated Mnase-seq and ChIP-seq profiling at mononucleosomal resolution for H3K14 acetylation.
Project description:We investigated natural inter-individual variation of the epigenome in a quantitative manner. To probe the degree of natural epigenomic diversity in S. cerevisiae, we compared three unrelated wild strains using replicated Mnase-seq and ChIP-seq profiling at mononucleosomal resolution for five histone marks (H3K4me3, H3K9ac, H3K14ac and H4K12ac and H3K4me1).
Project description:Comparison of the transcriptome profiles of a widely commercialized maize MON810 variety and its non-GM near-isogenic counterpart subjected to low N fertilization farming practices
Project description:Chromatin from Hnf1a-/- and wild-type mouse hepatocytes was immunoprecipitated with antibodies anti-H3K4me2 (Upstate 07-030). Amplified and labelled ChIP DNA and input DNA were hybridyzed to Mouse PromoterChip 5A.0 arrays.
Project description:Chromatin from mouse hepatocytes was immunoprecipitated with antibodies against Hepatocyte Nuclear Factor 4 alpha (Hnf4a) and IgG. Amplified and labelled ChIP DNA and input DNA were hybridyzed to Mouse PromoterChip 5A.1 arrays.
Project description:Chromatin from mouse hepatocytes was immunoprecipitated with anibodies anti-Hnf1 (SC, recognizes both Hnf1alpha and Hnf1beta). Amplified and labelled ChIP DNA and input DNA were hybridyzed to Mouse PromoterChip 5A.0 arrays.
Project description:Transcription profiling of wild type yeast strain as well as strains carrying a deletion of Gcn4, Arr1 or both. Gene expression in rich medium (YPD) and under osmotic stress conditions (YPD + 0.8M NaCl) was compared.
Project description:We characterized the genomes of five commonly used hematopoietic cell lines: Kasumi-1, REH, U-937, BV-173 and ME-1. DNA samples from all five cell lines were compared to commercial sex-matched DNA.