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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Saccharomyces cerevisiae Gis1 overexpression time course

ABSTRACT: The change of genome-wide transcription profiles as a result of Gis1 overexpression is monitored by comparing the transcriptomes isolated from cells where Gis1 overexpression is switched on or off. GIS1 was cloned into pCM190 vector under the control of the tetO7 promoter. The promoter is switched on when there is no doxycycline but off with doxycycline (20ug/ml). Cells were grown in medium with doxycycline, harvested, washes twice in sterile water, resuspended in the same medium with doxycycline (Dox+) or without doxycycline (dox-) and grown for additional 6 hours. Samples were taken for each condition at 3 and 6 hours. Time 0 sample was taken before resuspension.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Nianshu Zhang

PROVIDER: E-MEXP-2354 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The transcription activity of Gis1 is negatively modulated by proteasome-mediated limited proteolysis.

Zhang Nianshu N Oliver Stephen G SG

The Journal of biological chemistry 20091218 9

The transcriptional response to environmental changes has to be prompt but appropriate. Previously, it has been shown that the Gis1 transcription factor is responsible for regulating the expression of postdiauxic shift genes in response to nutrient starvation, and this transcription regulation is dependent upon the Rim15 kinase. Here we demonstrate that the activity of Gis1 is negatively modulated by proteasome-mediated limited proteolysis. Limited degradation of Gis1 by the proteasome leads to ...[more]

PMID: 20022953

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