Project description:This study newly identified Tripelennamine (TA) as an inhibitor of yeast meiosis and sporulation. To examine if and how exposure of sporulating yeast cells to TA changes the meiotic transcriptional program cells were sporulated for 0, 4, and 8 hours in the presence or absence of 100 uM TA.

2010-03-31 | E-MEXP-2522 | biostudies-arrayexpress

Antibody microarray profiling of pancreatic cancer using 12 urine samples

Project description:Antibody microarray based profiling of twelve urine samples. 3 healthy female 3 heatlhy male 3 female with pancreatic cancer 3 male with pancreatic cancer

2012-05-03 | E-MEXP-3006 | biostudies-arrayexpress

alternative promoter array HeLa HIF1 ChIP-chip

Project description:DNA binding profiling of endogenous HIF1A on proximal promoters in human HeLa cells exposed to 1% oxygen (hypoxia), using normoxic cells (21% oxygen) as reference. Biological background: The Hypoxia Inducible Factor Family of transcription factors is proposed as the main orchestrator of the cellular response to hypoxia. HIFs are heterodimers of a HIF alpha and a HIF beta subunit. HIF alpha protein stability is regulated by oxygen-dependent proteasomal degradation, and hence HIFs are strongly stabilized in hypoxia. Purpose of the study: a number of HIF1 ChIP-chip studies have been reported, employing various cell types, array platforms and HIF antibodies, and the overlap of HIF binding locations in these studies is relatively small. The aim of this study was to characterize HIF1 binding in an additional cell line (HeLa), and employing a different HIFalpha antibody. Experimental design: We conducted a total of six hybridizations employing four biological replicates. For two biological replicates, we performed dye-swap technical replicate experiments. Results summary: We identified 55 HIF binding locations in HeLa cells (FDR<2%). While this number is relatively low compared to previous studies, presumably due to limiting antibody sensitivity, the overlap with data from other cell lines is comparable to our HeLa data.

2012-05-20 | E-MEXP-3499 | biostudies-arrayexpress

Transcription profiling of Streptomyces coelicolor wild type and delta0877 and delta7173 mutant strains

Project description:Gene expression analysis of S. coelicolor M145 wild type strain, and mutants delta0877 and delta 7173. RNA samples were extracted from 48 h culture samples during growth in flasks in defined mineral medium MG supplemented with 5% of yeast extract. Cy5 labelled genomic DNA was used as the common reference.

2009-04-17 | E-MEXP-2132 | biostudies-arrayexpress

Global transcriptome profiling of sigH overexpression in L. sakei

Project description:Comparison of L. sakei overexpressing sigH and wild type strain

2012-01-01 | E-MEXP-3238 | biostudies-arrayexpress

Comparative Genomics of Urinary Tract Infectious Escherichia coli

Project description:Comparative genomic analysis of a range of urinary tract and urosepsis E.coli isolates

2011-05-20 | E-MEXP-3089 | biostudies-arrayexpress