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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human HeLa cells treated by various doses of cortisol

ABSTRACT: Glucocorticoids act through the glucocorticoid receptor (GR), a ligand activated transcription factor, to modulate gene expression profiles in target cells and tissues. The principal physiological glucocorticoid in humans, cortisol, is released in a pulsatile fashion from the adrenal gland, which results in oscillations in blood concentrations, with a period of 1 - 2 hours. The biological consequence of fluctuating cortisol concentrations has not been explored. To identify the role of cortisol concentration oscillations for target cell response a flow-through cell culture system was developed. Transcription profiling was performed in the absence and presence of cortisol. Cells were exposed to constant cortisol levels of 100ng/ml or 200 ng/ml or oscillating cortisol concentrations.

ORGANISM(S): Homo sapiens

SUBMITTER: Leo Zeef

PROVIDER: E-MEXP-2447 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Ultradian cortisol pulsatility encodes a distinct, biologically important signal.

McMaster Andrew A Jangani Maryam M Sommer Paula P Han Namshik N Brass Andy A Beesley Stephen S Lu Weiqun W Berry Andrew A Loudon Andrew A Donn Rachelle R Ray David W DW

PloS one 20110118 1

<h4>Context</h4>Cortisol is released in ultradian pulses. The biological relevance of the resulting fluctuating cortisol concentration has not been explored.<h4>Objective</h4>Determination of the biological consequences of ultradian cortisol pulsatility.<h4>Design</h4>A novel flow through cell culture system was developed to deliver ultradian pulsed or continuous cortisol to cells. The effects of cortisol dynamics on cell proliferation and survival, and on gene expression were determined. In add ...[more]

PMID: 21267416

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