Project description:Comparison of gene content between R. solanacearum strains GMI1000, NCPPB332, GMI1000::NCPPB332 recombinants, CFBP2968 and GMI1000::CFBP2968 recombinants
Project description:Cy3 and Cy5 direct labelled RNA from Bloodstream MiTat1.1 trypanosomes and Procyclic 427 Lister were hybridized onto JCVI Trypanosoma brucei oligoarrays (version2). Procyclic RNA were used as control for data analysis.
Project description:To generate differential gene expression profiles across the moult cycle of Antarctic krill, relative to an early intermoult stage.
Project description:Gene expression analysis of S. coelicolor M145 wild type strain, and mutants delta0877 and delta 7173. RNA samples were extracted from 48 h culture samples during growth in flasks in defined mineral medium MG supplemented with 5% of yeast extract. Cy5 labelled genomic DNA was used as the common reference.
Project description:Control (Col-0) and mutants (rcd1-1, rcd1-3, rcd1-4 and sro1-1) were grown for 22 days under control conditions. Whole rosettas were harvested and analyzed for changes in gene expression between Col-0 and the mutants.
Project description:Bacterial Gre factors associate with RNA polymerase (RNAP) and stimulate intrinsic cleavage of the nascent transcript at the active site of RNAP. Biochemical and genetic studies to date have shown that E. coli Gre factors prevent transcriptional arrest during elongation and enhance transcription fidelity. Furthermore, Gre factors participate in stimulation of promoter escape and suppression of promoter-proximal pausing during beginning of RNA synthesis in E. coli. Although Gre factors are conserved in general bacteria, limited functional studies have been performed in bacteria other than E. coli. In this investigation, ChAP-chip analysis was conducted to visualize the distribution of B. subtilis GreA on the chromosome and determine the effects of GreA inactivation on core RNAP trafficking. Our data show that GreA is uniformly distributed in the transcribed region from the promoter to coding region with core RNAP, and its inactivation induces RNAP accumulation at many promoter or promoter-proximal regions. Based on these findings, we propose that GreA would constantly associate with core RNAP during transcriptional initiation and elongation, and resolves its stalling at promoter or promoter-proximal regions, thus contributing to the even distribution of RNAP along the promoter and coding regions in B. subtilis cells.
Project description:Gene expression profiling of TRAMP-C2, transgenic adenocarcinoma of mouse prostate, cell line. The experiment was done to see which genes were over- or underexpressed in the cell line. The results were combined with aCGH results of the same samples (experiment Gene copy number profiling of TRAMP-C2 cell line using aCGH) to see, whether the copy number status had any effect on gene expression.