Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

95 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Bone marrow derived dendritic cells (BMDC) and MDP and MSU

ABSTRACT: Muramyl dipeptide (MDP) and Monosodium urate crystals (MSU) promote a synergistic effect on NOD2 and NLRP3 with a unique transcriptional profile in murine dendritic cells

ORGANISM(S): Mus musculus

SUBMITTER: alessandra mortellaro

PROVIDER: E-MEXP-2905 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

NLRP3 inflammasome affects DNA damage responses after oxidative and genotoxic stress in dendritic cells

Project description:Effect of Monosodium Urate Crystals in wild-type and NLRP3 ko murine dendritic cells

2013-07-11 | E-MEXP-3858 | biostudies-arrayexpress

Expression analysis of human PBMCs stimulated with Borrelia, MDP or Pam3cys.

Project description:Analysis examining the effect of the Crohn's disease-associated ATG16L1 polymorphism on the responses to stimulation components derived from various bacteria classes. Expression analysis was performed on RNA extracted from peripheral blood mononuclear cells (PBMCs) from healthy genotyped volunteers were stimulated for 24h with RPMI alone, or RPMI containing heat-killed Borrelia burgdorferi , muramyl dipeptide (MDP) or Pam3Cys.

2015-06-30 | E-GEOD-68765 | biostudies-arrayexpress

Study of transcriptional effects of monosodium urate crystal (MSU) on HaCaT keratinocyte cell line.

Project description:Aim of the study was to characterize at a molecular level (changes in transcriptomes) the effect of monosodium urate crystal (MSU) on HaCaT keratinocyte cell line. This was adressed by using a culture model. The HaCaT cell line (human keratinocytes) was stimulated by MSU (1mg/mL) vs control for 12 hrs. By using genome-wide expression profiling, we identified deregulation of functionally relevant gene networks. HaCaT were obtained from Cell Lines Service (Eppelheim, Germany) and grown in DMEM medium (PAN biotech, Aidenbach, Germany) supplemented with 10% FBS (Life Technology, Grand Island, NY, USA), L-glutamine and non-essential amino acid. Before the treatment HaCaT cells were cultured in serum-free medium for 12hrs. HaCaT were treated with MSU (1mg/ml) vs DMEM control for 12hrs then submitted to RNA extration and gene expression profiling. Triplicate experiments were performed: HaCaT control (n=3), MSU-treated (n=3).

2015-10-29 | E-GEOD-65931 | biostudies-arrayexpress

Immune response eQTL study in primary human monocytes stimulated with multiple microbial ligands under multiple time points

Project description:Gene expression arrays and genotyping data in primary human monocytes from healthy individuals of European ancestry that have either been unstimulated (baseline), exposed to Lipopolysaccharide (90 minutes or 6 hours duration), muramyl-dipeptide (90 minutes or 6 hours duration) or transfected with 5’-triphosphate RNA (90 minutes or 6 hours duration). Please note that eQTL analysis methods and result files format are available as additional files attached to this experiment ( see https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5631/files/ ).

2017-05-16 | E-MTAB-5631 | biostudies-arrayexpress

MDP phosphorylation by NAGK is essential for NOD2 activation

Project description:Bacterial cell wall components provide various unique molecular structures that are detected by pattern recognition receptors (PRRs) of the innate immune system as non-self. Most bacterial species form a cell wall that consists of peptidoglycan, a polymeric structure comprising of alternating amino sugars. Muramyl dipeptide (MDP) is the minimal, biologically active molecular structure derived from peptidoglycan, and its immunogenicity is well documented in numerous studies. MDP is sensed by the cytosolic nucleotide-binding and nucleotide oligomerization domain-like receptor 2 (NOD2), which triggers a pro-inflammatory response upon engagement 1,2. Conducting a forward genetic screen to identify factors required for MDP detection, we discovered that N-acetylglucosamine kinase (NAGK) is essential for the immunostimulatory activity of MDP. NAGK, which has previously been identified to contribute to the hexosamine salvage pathway in humans, is broadly expressed and inducible in innate immune cells. Mechanistically, NAGK functions upstream of NOD2 in that it directly phosphorylates the N-acetylmuramic moiety of MDP at the hydroxyl group of its C6 position, yielding 6-O-phospho-MDP. NAGK-phosphorylated MDP constitutes an essential component of NOD2 dependent signal transduction. Altogether, these results unveil a previously unknown interconnection of amino sugar metabolism and innate immunity to bacterial cell walls.

2022-06-13 | PXD022384 | Pride

Transcription profiling of mouse naive and tolerant macrophages treated with MDP- and Pam3CSK4

Project description:Most of the genes were self-tolerized by Pam3CSK4 and MDP but there was no or minimal cross-tolerization. The transcriptome induced via Nod2 stimulation is greatly expanded in TLR2-tolerant macrophages. Experiment Overall Design: Genes induced by Pam3CSK4 or MDP were selected based on a three-fold increase over expression levels in unstimulated macrophages. Tolerized genes are defined as genes downregulated more than 2-fold in tolerant macrophages stimulated with Pam3CSK4 or MDP.

2008-04-05 | E-GEOD-10182 | biostudies-arrayexpress

Multimodal smart systems reprMultimodal smart systems reprogram macrophages and remove urate to treat gouty arthritisogram macrophages and remove urate to treat gouty arthritis

Project description:Gouty arthritis (GA) is a chronic and progressive disease that often develops due to high urate levels, leading to joint damage and characterized by an inflammatory immune microenvironment (IIMe). Quantitative proteomics further revealed D-N camouflaged with hybid membranes of M2 exosome and its parent microphage (D-N[EM2]) treated GA disease mechanisms.

2024-07-02 | PXD051823 | Pride

Transcriptomic analysis of synovial fibroblasts from monosodium urate crystal-induced arthritis with or without I-BET151 treatment.

Project description:We used I-BET 151, an isoxazoloquinoline that specifically inhibits interaction of BET proteins with acetylated histones to restrict inflammatory tissue priming in a mouse model of iterated monosodium urate (MSU) crystal-induced arthritis. Systemic administration of I-BET 151 abolished the enhancement of arthritis upon repeated injection of MSU crystals.

2022-02-17 | GSE196679 | GEO

Reprogramming of primary human Philadelphia chromosome-positive B cell acute lymphoblastic leukemia cells into nonleukemic macrophages

Project description:BCR–ABL1+ precursor B-cell acute lymphoblastic leukemia (BCR– ABL1+ B-ALL) is an aggressive hematopoietic neoplasm characterized by a block in differentiation due in part to the somatic loss of transcription factors required for B-cell development. We hypothesized that overcoming this differentiation block by forcing cells to reprogram to the myeloid lineage would reduce the leukemogenicity of these cells. We found that primary human BCR–ABL1+ B-ALL cells could be induced to reprogram into macrophage-like cells by exposure to myeloid differentiation-promoting cytokines in vitro or by transient expression of the myeloid transcription factor C/EBPα or PU.1. The resultant cells were clonally related to the primary leukemic blasts but resembled normal macrophages in appearance, immunophenotype, gene expression, and function. Most importantly, these macrophage-like cells were unable to establish disease in xenograft hosts, indicating that lineage reprogramming eliminates the leukemogenicity of BCR–ABL1+ B-ALL cells, and suggesting a previously unidentified therapeutic strategy for this disease. Finally, we determined that myeloid reprogramming may occur to some degree in human patients by identifying primary CD14+ monocytes/ macrophages in BCR–ABL1+ B-ALL patient samples that possess the BCR–ABL1+ translocation and clonally recombined VDJ regions. We obtained the expression profiles of 5 human B-ALL samples using Afymmetrix U133A2 microarrays. Blasts were either analyzed without culture, or cultured in the presence of myeloid cytokines and sorted into CD14+ and CD19+ populations.

2015-04-01 | E-GEOD-66792 | biostudies-arrayexpress