Project description:microRNA expression profiles in two stage-specific populations of oligodendrocytes: OPCs expressing the A2B5 ganglioside (A2B5+ cells) and OLs that are positive for the galactocerebroside marker (GalC+ cells). Cells were isolated postnatal day 7.
Project description:Human embryonic stem cell lines HSF-1 and HSF-6 were differentiated on human fetal gonadal stromal cells for 7 days. The cells were labelled with antibodies against cKIT and SSEA1 and the double positive and double negative cells were collected for microarray analysis.
Project description:Human fetal dissociates from 19-22 week gestational age were magnetically sorted for CD140a antigen. CD140a-defined OPCs were plated into serum free conditions and allowed to differentiate in the absence of growth factors or mitogens. RNA was extracted from cells immediately following isolation and every day for 4 days. To block differentiation, matched cells were cultured in the presence of PDGF-AA (10ng/ml). This treatment prevents the acquisition of O4-positive oligodendrocyte cell fate and delays MBP mRNA expression by human CD140a-sorted OPCs. 29 samples, 4 time points, 2 media conditions, at least three biological replicates per time point and media condition
Project description:Parallel transcription profiling of cardiomyocyte clusters derived from human embryonic stem cells. Both mRNA (E-MEXP-2654) and miRNA (this experiment) transcription are measured and fetal and adult heart tissues are included as controls. This experiment was updated on 7th July 2011 to fix incorrect AH6 and FH3 files
Project description:miRNA expression profiling of prostate cancer cell lines, PC-3, DU145, LAPC-4, VCaP, LNCaP, 22rv1, and normal prostate epithelial cells, PrECs, was done after treating the cells with DNA demethylating agent 5-aza-2'-deoxycytidine (5azadC; Sigma-Aldrich, St. Louis, MO) and histone deacetylase inhibitor trichostatin A (TSA; Sigma-Aldrich). These treatments relieve epigenetic modifications, and thus reveal potentially epigenetically silenced miRNAs amongst the miRNAs with increased expression after the treatments.