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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human embryonic stem cell lines HSF-1 and HSF-6 differentiated on human fetal gonadal stromal cells selected for cKIT and SSEA1 antibodies

ABSTRACT: Human embryonic stem cell lines HSF-1 and HSF-6 were differentiated on human fetal gonadal stromal cells for 7 days. The cells were labelled with antibodies against cKIT and SSEA1 and the double positive and double negative cells were collected for microarray analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Amander Clark

PROVIDER: E-MEXP-1946 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Derivation of primordial germ cells from human embryonic and induced pluripotent stem cells is significantly improved by coculture with human fetal gonadal cells.

Park Tae Sub TS Galic Zoran Z Conway Anne E AE Lindgren Anne A van Handel Benjamin J BJ Magnusson Mattias M Richter Laura L Teitell Michael A MA Mikkola Hanna K A HK Lowry William E WE Plath Kathrin K Clark Amander T AT

Stem cells (Dayton, Ohio) 20090401 4

The derivation of germ cells from human embryonic stem cells (hESCs) or human induced pluripotent stem (hIPS) cells represents a desirable experimental model and potential strategy for treating infertility. In the current study, we developed a triple biomarker assay for identifying and isolating human primordial germ cells (PGCs) by first evaluating human PGC formation during the first trimester in vivo. Next, we applied this technology to characterizing in vitro derived PGCs (iPGCs) from plurip ...[more]

PMID: 19350678

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