Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

37 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Response of Streptomyces coelicolor to treatment with the antibiotics vancomycin bacitracin and moenomycin A

ABSTRACT: To determine the response at the transcriptional level of S. coelicolor cells to treatment with 3 antibiotics that target distinct stages of cell wall biosynthesis, biological triplicate cultures of strain M600 were treated with sub-lethal concentrations (10 µg/ml) of vancomycin, bacitracin or moenomycin A. Samples were taken 0, 30, 60, 90 minutes after addition of the antibiotic. A negative control which received no drug treatment was also performed.

ORGANISM(S): Streptomyces coelicolor A3(2)

SUBMITTER: Andrew Hesketh

PROVIDER: E-MEXP-3032 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Genome-wide dynamics of a bacterial response to antibiotics that target the cell envelope.

Hesketh Andy A Hill Chris C Mokhtar Jehan J Novotna Gabriela G Tran Ngat N Bibb Mervyn M Hong Hee-Jeon HJ

BMC genomics 20110511

<h4>Background</h4>A decline in the discovery of new antibacterial drugs, coupled with a persistent rise in the occurrence of drug-resistant bacteria, has highlighted antibiotics as a diminishing resource. The future development of new drugs with novel antibacterial activities requires a detailed understanding of adaptive responses to existing compounds. This study uses Streptomyces coelicolor A3(2) as a model system to determine the genome-wide transcriptional response following exposure to thr ...[more]

PMID: 21569315

Similar Datasets

Transcriptional profiling of the sigma factor sigE deletion mutant of Streptomyces coelicolor (J2130) at 0, 30, 60 and 90 minutes after treatment with sub-lethal concentration of vancomycin.

Project description:SigE is a sigma factor found in Streptomyces species and is the key regulator of cell envelope stress response in Streptomyces coelicolor. To determine the regulon of SigE under conditions of cell envelope stress, 10 ug / ml vancomycin was used to induce cell envelope stress in the wild type and a sigE deletion strain of S. coelicolor. Samples were taken 0, 30, 60 and 90 minutes after addition of vancomycin and subjected to transcriptional profiling using Affymetrix arrays. Note – equivalent data for wild type Streptomyces coelicolor M600 is submitted as part of E-MEXP-3032. The data for both M600 (E-MEXP-3032) and J2130 (this submission) were generated at the same time employing the same protocols.

2019-04-24 | E-MTAB-7814 | biostudies-arrayexpress

Transcriptional profiling of the developmental gene whiG deletion mutant of Streptomyces venezuelae ATCC 10712 at 7 time points from 8 to 20 hours of growth.

Project description:WhiG is a sigma factor found in Streptomyces species. It has a central role in sporulation in these bacteria. Although mutants in whiG are able to form aerial mycelia, these fail to differentiate into spores. The aim of this transcription profiling experiment was to measure genome wide transcript levels in the wild type and the whiG deletion mutant at 7 time points from 8 to 20 hours during the growth cycle of Streptomyces venezuelae.

2019-07-12 | E-MTAB-8114 | biostudies-arrayexpress

Transcription profiling of Streptomyces coelicolor bldC null mutant and parental strain M600

Project description:Transcription profiling of Streptomyces coelicolor bldC null mutant and parental strain M600 in liquid culture exponential phase

2012-03-31 | E-MEXP-3197 | biostudies-arrayexpress

Transcription profiling by array of Streptomyces coelicolor bldD null mutant and parental strain M600

Project description:Comparison transcriptional profile of S. coelicolor M600 (a plasmid-free derivative of the wild type) with that of a congenic bldD null mutant in rapidly growing cultures in liquid rich medium.

2010-08-05 | E-MEXP-2853 | biostudies-arrayexpress

Transcriptional profiling of the developmental gene whiB deletion mutant of Streptomyces venezuelae ATCC 10712 at 7 time points from 8 to 20 hours of growth

Project description:WhiB is the founding member of a family of proteins (the WhiB-like [Wbl] family) that carry a [4Fe-4S] iron-sulfur cluster and play key roles in diverse aspects of the biology of actinomycetes, including pathogenesis, antibiotic resistance, and the control of development. In Streptomyces, WhiB is essential for the process of developmentally controlled cell division that leads to sporulation. The aim of this transcription profiling experiment was to measure genome wide transcript levels in the wild type and the whiB deletion mutant at 7 time points from 8 to 20 hours during the growth cycle of Streptomyces venezuelae.

2021-06-30 | E-MTAB-10631 | biostudies-arrayexpress

Transcriptional profiling of the developmental gene whiA deletion mutant of Streptomyces venezuelae ATCC 10712 at 7 time points from 8 to 20 hours of growth.

Project description:WhiA is an unusual transcriptional regulator found in Streptomyces species related to eukaryotic homing endonucleases. It plays a central role in sporulation in these bacteria. The aim of this transcription profiling experiment was to measure genome wide transcript levels in the wild type and the whiA deletion mutant at 7 time points from 8 to 20 hours during the growth cycle of Streptomyces venezuelae.

2020-12-11 | E-MTAB-9728 | biostudies-arrayexpress

High-resolution mass spectrometry based proteomic analysis of the response to vancomycin-induced cell wall stress in Streptomyces coelicolor A3(2)

Project description:Understanding how bacteria survive periods of cell wall stress is of fundamental interest and can help generate ideas for improved antibacterial treatments. In this study we use tandem mass tagging to characterize the proteomic response of vancomycin resistant Streptomyces coelicolor to the exposure to sub-lethal levels of the antibiotic. A common set of 804 proteins were identified in triplicate experiments. Contrasting changes in the abundance of proteins closely associated with the cytoplasmic membrane with those taking place in the cytosol identified aspects of protein spatial localization that are associated with the response to vancomycin. Enzymes for peptidoglycan precursor, mycothiol, ectoine and menaquinone biosynthesis together with a multisubunit nitrate reductase were recruited to the membrane following vancomycin treatment. Many proteins with regulatory functions (including sensor protein kinases) also exhibited significant changes in abundance exclusively in the membrane associated protein fraction. Several enzymes predicted to be involved in extracellular peptidoglycan crossbridge formation became significantly depleted from the membrane. A comparison with data previously acquired on the changes in gene transcription following vancomycin treatment identified a common high-confidence set of changes in gene expression. Generalized changes in protein abundance indicate roles for proteolysis, the pentose phosphate pathway and a reorganization of amino acid biosynthesis in the stress response.

2018-05-15 | PXD002216 | Pride

Transcriptional response of Pseudomonas aeruginosa PAO1 to human host defense peptide LL-37

Project description:In this experiment the transcriptional response of the opportunistic human pathogen Pseudomonas aeruginosa towards physiological concentrations of the major human host defense peptide LL-37 was investigated using microarrays. To this aim, three independent cultures of P. aeruginosa PAO1 were grown until mid-log phase in Mueller-Hinton broth and subsequently incubated with either sublethal LL-37 concencentrations (20 M-5g/ml) or without peptide for 2 h at 37 M-0C following RNA extraction and microarray analysis.

2013-12-20 | E-MEXP-3970 | biostudies-arrayexpress

Transcriptional profiling of Pseudomonas aeruginosa PAO1 strain and its delta pppA-ppkA mutant growing under non-stress conditions and conditions of oxidative stress.

Project description:Effect of deletion of pppA and ppkA genes in Pseudomonas aeruginosa on global transcriptome of mutant strain and effect of this deletion on response to oxidative stress.

2012-01-01 | E-MEXP-3117 | biostudies-arrayexpress

Transcription profiling of Drosophila allowed to evolve a defense response to infection to Pseudomonas aeruginosa over 10 generations

Project description:Drosophila harbor substantial genetic variation for antibacterial defense. We allowed wild-caught Drosophila melanogaster to evolve a defense response to systemic infection with the human opportunistic pathogen, Pseudomonas aeruginosa over 10 generations. We performed genome wide transcriptional profiling in selected lines relative to control lines (not infected, but were exposed to the same bottleneck in population size as their paired selected lines by randomly selecting a set of individuals to found the next generation, and then infected at the end of the 10 generations), to identify specifically the genetic basis of the evolved immune response.

2009-02-20 | E-MEXP-2054 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data