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Glucose depletion inhibits translation initiation in yeast - polysome and monosome profiling

ABSTRACT: This is a polysome/monosome profiling study on yeast undergoing glucose depletion. Polysomes and monosomes are extracted and the transcripts bound to each fraction are measured by microarray. The study reveals that glucose depletion inhibits translation initiation in a mechanism involving eIF4A loss and 48S pre-initiation complex accumulation, while the pentose phosphate pathway is co-ordinately up-regulated

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Leo Zeef

PROVIDER: E-MEXP-3038 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Glucose depletion inhibits translation initiation via eIF4A loss and subsequent 48S preinitiation complex accumulation, while the pentose phosphate pathway is coordinately up-regulated.

Castelli Lydia M LM Lui Jennifer J Campbell Susan G SG Rowe William W Zeef Leo A H LA Holmes Leah E A LE Hoyle Nathaniel P NP Bone Jonathon J Selley Julian N JN Sims Paul F G PF Ashe Mark P MP

Molecular biology of the cell 20110727 18

Cellular stress can globally inhibit translation initiation, and glucose removal from yeast causes one of the most dramatic effects in terms of rapidity and scale. Here we show that the same rapid inhibition occurs during yeast growth as glucose levels diminish. We characterize this novel regulation showing that it involves alterations within the 48S preinitiation complex. In particular, the interaction between eIF4A and eIF4G is destabilized, leading to a temporary stabilization of the eIF3-eIF ...[more]

PMID: 21795399

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