Transcriptional profiling by array to investigate the effect of interfacial stress on rat alveolar type II cell signalling and gene expression (conventional air-liquid interface)

ABSTRACT: Many epithelia have contact with air-liquid interfaces. This applies particularly to the lung, where one of the epithelial cell types (the surfactant secreting alveolar type II [AT II] cells) even project into the air. This specific environment may be of considerable physiological relevance; however, only few data exist to provide a satisfying description. This is mainly due to the difficulty to manipulate cell-air contacts in a specific way. In previous investigations, using new microscopic approaches, we found that the presence of an air-liquid interface leads to a paradoxical situation: it is a potential threat that may cause cell injury, but also a potent stimulus for the cells: AT II cells respond promptly, and show sustained Ca2+-signals that activate exocytosis. In this report, gene

ORGANISM(S): Rattus norvegicus

SUBMITTER: Nina Hobi 

PROVIDER: E-MEXP-3363 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress