Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

36 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Calcitriol triggers axogenesis and myelination

ABSTRACT: Using pangenomic cDNA microarrays and qPCR techniques, we identified the genes regulated by calcitriol (1,25 (OH)D3, 10 nM) in dorsal root ganglia and/or Schwann cells. After 24 hours of calcitriol supplementation, we found a modified expression of many genes involved in axogenesis and myelination.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Stephane Girard

PROVIDER: E-MEXP-3491 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

BIN-67 small cell ovarian carcinoma Expression array

Project description:The biology of small cell ovarian carcinoma of the hypercalcemic type (SCCOHT), which is a rare and aggressive form of ovarian cancer, is poorly understood. Tumourigenicity, in vitro growth characteristics, genetic and genomic anomalies, and sensitivity to standard and novel chemotherapeutic treatments were investigated in the unique SCCOHT cell line, BIN-67, to provide further insight in the biology of this rare type of ovarian cancer. Microarray expression analysis was performed using the GeneChip? Human Genome U133 Plus 2.0 Array (Affymetrix?, Santa Clara, CA) with total RNA from BIN-67 cells. Hybridization and scanning were performed at the McGill University and Genome Quebec Innovation Centre (Montreal, QC). Gene expression levels were determined from the scanned images using Affymetrix? Microarray Suite (MAS) version 5.0 software expression algorithm (Affymetrix?, Santa Clara, CA). Gene expression profiles were compared with Affymetrix U133 Plus 2.0 (MAS5.0) generated expression profiles (similarly normalized) from 10 human normal ovarian surface epithelial (OSE) cell brushings available in the ArrayExpress database (www.ebi.ac.uk/arrayexpress/), accession number E-GEOD-18520.

2013-09-24 | E-MEXP-3872 | biostudies-arrayexpress

Transcription profiling of two mesothelioma cell lines (MPP89 and REN), and of HMC-TERT non tumoral mesothelial cells

Project description:miRNA profile analysis of two mesothelioma cell lines (MPP89 and REN), and of HMC-TERT non tumoral mesothelial cells

2008-10-09 | E-MEXP-1699 | biostudies-arrayexpress

Transcription profiling of human lung adenocarcinoma A549 cells treated with vehicle (DMSO) or Etoposide (ETOP, 10 uM ), Benzo(a)pyrene Cryptolepineand 2-Amino-1-methyl-6-phenylimidazo [4,5-b]pyridine for 6 h, 16 h, 24 h, 48 h and 72 h

Project description:Title: Prediction of cell cycle behaviour in response to DNA damage agents via transcriptomic approach <br/> Description: Human lung adenocarcinoma A549 cells were treated with vehicle (DMSO) or Etoposide (ETOP, 10 M-BM-5M ), Benzo(a)pyrene (BaP, 25 M-BM-5M ), Cryptolepine ( CLP, 2.5 M-BM-5M) and 2-Amino-1-methyl-6-phenylimidazo [4,5-b]pyridine (PhIP, 50 M-BM-5M) for 6 h, 16 h, 24 h, 48 h and 72 h.

2006-06-01 | E-MIMR-102 | biostudies-arrayexpress

HEK293lines_Human_Exon_1.0_ST_arrays_exondata

Project description:Comparison of transcriptional profiles of 6 different HEK293 cell line variants, at the exon-level (extended set) and gene-level (core set)

2013-07-16 | E-MEXP-3516 | biostudies-arrayexpress

Transcription profiling by array of human renal proximal tubular cell cultures treated with nephrotoxins

Project description:This experiment is designed to investigate common transcriptional alterations in renal proximal tubular cell cultures due to nephrotoxin exposure.

2011-02-01 | E-MEXP-2599 | biostudies-arrayexpress

microRNA expression in CagA-expressing cells

Project description:Comprehensive analysis of microRNA expression were performed with 238 rat genes of microRNA between CagA-expressing and -non-expressing cells. CagA expression was controlled by tetracycline-off system in RGM1 cells. In the study presented here, RGM1 cells were used to acquire expression profiles of 238 microRNA genes.

2011-04-21 | E-GEOD-27009 | biostudies-arrayexpress

Transcription profiling of human mesothelioma cells MPP-89 REN compared to mesothelial cells HMC-TERT

Project description:gene expression profile of mesothelioma cells (MPP-89; REN) compared to mesothelial cells (HMC-TERT)

2008-10-09 | E-MEXP-1701 | biostudies-arrayexpress

Transcription profiling of human mesothelioma cells (MPP-89, REN) compared to mesothelial cells (HMC-TERT)

Project description:gene expression profiles of mesothelioma cells (MPP-89, REN) compared to mesothelial cells (HMC-TERT)

2008-10-09 | E-MEXP-1700 | biostudies-arrayexpress

BIN-67 small cell ovarian carcinoma SNP array

Project description:The biology of small cell ovarian carcinoma of the hypercalcemic type (SCCOHT), which is a rare and aggressive form of ovarian cancer, is poorly understood. Tumourigenicity, in vitro growth characteristics, genetic and genomic anomalies, and sensitivity to standard and novel chemotherapeutic treatments were investigated in the unique SCCOHT cell line, BIN-67, to provide further insight in the biology of this rare type of ovarian cancer. Chromosomal anomalies in BIN-67 cells were inferred using the Infinium? genotyping technology with the HumanHap300-Duo Genotyping BeadChip (Illumina, San Diego, CA, USA). This BeadChip contains about 318,000 genetic markers within approximately a 5 kb median SNP spacing. Genotyping and imaging using BeadStudio Data Analysis software (Illumina, San Diego, CA, USA) were performed at the McGill University and Genome Quebec Innovation Centre (Montreal, QC).

2013-09-24 | E-MTAB-1584 | biostudies-arrayexpress

An impaired respiratory electron chain triggers down-regulation of the energy metabolism and de-ubiquitination of solute carrier amino acid transporters

Project description:We integrated metabolome and proteome profiles of the parental cell line 143B.TK- versus ρ0, including PTM analyses such as phosphorylation and ubiquitination to characterize the impact of the absence of mtDNA for the entire cell. For quantitative proteome profiling, we used a shotgun LC-MS/MS approach including the classical SILAC labeling. For comprehensive metabolome profiling, we applied a targeted LC-MS approach, based on multiple reaction monitoring (MRM).</br></br>Our study revealed that mtDNA depletion leads to a non-uniform down-regulation of the mitochondrial energy metabolism in ρ0 cells on the proteome level. Metabolites of the TCA cycle were highly dysregulated which in turn had an impact on the amino acid levels, which were up regulated. Perturbation of the mitochondrial energy metabolism could lead to an activation of the retrograde response, indicated by sets of up-regulated signaling pathways in ρ0 cells, further supported by altered phosphorylation in signaling pathways and the cytoskeleton as well as de-ubiquitination of SLC transporters.

2016-02-08 | PXD002425 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data