Project description:We analyzed and classified Whi3-regulated and ploidy-regulated genes in haploid and diploid strains of the Sigma1278b genetic background under vegetative growth conditions.<br><br>For this purpose, we measured transcriptional profiles of two different haploid MATa and one diploid MATa/a yeast strains of the following genotypes: WHI3 strain (SS_YHUM468=YHUM0468), whi3 strain (SS_ySS137=YHUM1920) and whi3-delta/whi3-delta strain (SS_ySS137dipl=YHUM2152). All three strains were grown in duplicate in YNB medium supplemented with tryptophan and uracil at 30 degrees C to an optical density of 1.0 before extraction of total RNA and transcriptional profiling<br><br>
Project description:Parasitoids were considered to have the ability to synthesize the lipid. The cotton aphids were parasitized by Lysiphlebia japonica, and Lysiphlebia japonica obtained lipids from cotton aphids. In our study, we get the 3 days larva and pupa from cotton aphids and analysis the expression of the genes involved in the lipid related pathway of these two developmental stages.
Project description:The early blood vessels of the embryo and yolk sac in mammals develop by aggregation of de novo forming angioblasts into a primitive vascular plexus, which then undergoes a complex remodeling process. Angiogenesis is also important for disease progression in the adult. However, the precise molecular mechanism of vascular development remains unclear. It is therefore of great interest to determine which genes are specifically expressed in developing endothelial cells.Here, we utilized Flk1-deficient mouse embryos, which lack endothelial cells, to perform a genome-wide survey for genes related to vascular development. Wild type (WT), Flk1+/GFP and Flk1 KO embryos proper (fetuses) were dissected out at 8.5 dpc. Total RNAs from these embryos were prepared using RNeasy kit. Gene expression analysis was performed by GeneSpring software.
Project description:Microarray analyses were used to compare changes in gene expression between two sets of environmental conditions ('normal' and 'dauer larva-inducing') and between isolates (N2, DR1350, RIL-14, RIL-17). These comparisons were made in two experimental blocks: block one N2 and DR1350; block two RIL-14 and RIL-17. For each isolate there were three biological replicates for both set environmental conditions.
Project description:Microarray analyses were used to compare changes in gene expression between two sets of environmental conditions ('normal' and 'dauer larva-inducing') and between isolates (N2, DR1350, RIL-14, RIL-17). These comparisons were made in two experimental blocks: block one N2 and DR1350; block two RIL-14 and RIL-17. For each isolate there were three biological replicates for both set environmental conditions.
Project description:We generated MOF, H4 and H4K16ac ChIP-seq experiments in D.melanogaster male and female wt. All experiments were performed with 3rd instard salivary glands biological material. Raw and processed data are provided here.
Project description:dTaf1025 deletion strain where the Taf10 and Taf10b genes are deleted. The animals were syncronized to spiracle eversion and then a mix of 10 larva were used for RNA extraction and hybridization.
Project description:The combination of peginterferon and ribavirin is the standard treatment for chronic hepatitis C. Our recent clinical study suggests that ribavirin augments the induction of interferon stimulated genes (ISGs) in patients treated for HCV infection [1]. In order to further characterize the mechanisms of action of ribavirin, we examined the effect of ribavirin treatment on ISG induction in cell culture. In addition, the effect of ribavirin on infectious HCV cell culture systems was also studied. Similar to interferon-alpha, ribavirin potently inhibits JFH-1 infection of Huh7.5.1 cells in a dose-dependent manner, which spans the physiological concentration of ribavirin in vivo. Microarray analysis and subsequent quantitative PCR assays demonstrated that ribavirin treatment resulted in the induction of a distinct set of ISGs. These ISGs, including IRF7 and IRF9 are known to play an important role in anti-HCV responses. When ribavirin is used in conjunction with interferon, induction of specific ISGs is synergistic when compared to either drug applied separately. Direct up-regulation of these antiviral genes by ribavirin is mediated by a novel mechanism different from those associated with interferon signaling and intracellular double stranded RNA sensing pathways such as RIG-I and MDA5. RNA interference studies excluded the activation of the Toll-like receptor and NF-KappaB pathways in the action of ribavirin. In conclusion, our study suggests that ribavirin, acting via a novel innate mechanism, potentiates the anti-HCV effect of interferon. Understanding the mechanism of action of ribavirin would be valuable in identifying novel antivirals. RNA from three samples were treated with Ribavirin and compared to three PBS treated samples