Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

47 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Kolsto-B. cereus Transcriptional response of the B. cereus ATCC14579 wild type to bile salts stress

ABSTRACT: Comparison of the transcriptional response of B. cereus ATCC14579 before and after 15 min bile salts stress.

ORGANISM(S): Bacillus cereus

SUBMITTER: Roger Simm

PROVIDER: E-MEXP-3535 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

BC4707 is a major facilitator superfamily multidrug resistance transport protein from Bacillus cereus implicated in fluoroquinolone tolerance.

Simm Roger R Vörös Aniko A Ekman Jaakko V JV Sødring Marianne M Nes Ingerid I Kroeger Jasmin K JK Saidijam Massoud M Bettaney Kim E KE Henderson Peter J F PJ Salkinoja-Salonen Mirja M Kolstø Anne-Brit AB

PloS one 20120516 5

Transcriptional profiling highlighted a subset of genes encoding putative multidrug transporters in the pathogen Bacillus cereus that were up-regulated during stress produced by bile salts. One of these multidrug transporters (BC4707) was selected for investigation. Functional characterization of the BC4707 protein in Escherichia coli revealed a role in the energized efflux of xenobiotics. Phenotypic analyses after inactivation of the gene bc4707 in Bacillus cereus ATCC14579 suggested a more spe ...[more]

PMID: 22615800

Similar Datasets

Kolsto-Bacillus cereus transcriptional response of ATCC14579 BC4707 mutant to bile salts stress

Project description:Analysis of the transcriptional response of B. cereus ATCC14579 BC4707 deletion mutant after 15 min bile salts stress

2012-01-31 | E-MEXP-3536 | biostudies-arrayexpress

Kolsto-B. cereus Comparison of WT and BC4707 mutant during growth in logarithmic phase

Project description:Comparison of B. cereus ATCC14579 wild type and its isogenic BC4707 mutant under growth in logarithmic phase

2012-01-31 | E-MEXP-3537 | biostudies-arrayexpress

Kolsto-Bacillus cereus comparison of wild type and bc4707 deletion mutant after Bile Salts stress

Project description:Comparison of wild type Bacillus cereus ATCC14579 and its isogenic bc4707 deletion mutant under logarithmic growth (OD=0.5)

2012-01-31 | E-MEXP-3529 | biostudies-arrayexpress

Gene transcription from the linear plasmid pBClin15 leads to cell lysis and extracellular DNA dependent aggregation of B. cereus ATCC 14579 in response to quinolone induced stress

Project description:Several members of the Bacillus cereus group of bacteria carry lysogenic phages of the family Tectiviridae. The Bacilus cereus reference strain (ATCC 14579) harbor a linear plasmid (pBClin15) that is highly similar to the genomes of the Tectiviruses. Production of active phages has however never been reported for pBClin15 and the role of pBClin15 in B. cereus physiology has been enigmatic. We have for the first time demonstrated an effect of pBClin15 on the physiology of B. cereus ATCC 14579 whereby the wild type is more sensitive to DNA damaging antibiotics compared to a pBClin15 cured strain. The microarray experiments in this study were designed to highlight transcriptional differences between the B. cereus ATCC 14579 wild type and a plasmid cured variant that potentially could explain the impact of pBClin15 on the B. cereus physiology. Microarray experiments were carried out under non-stressful conditions (growth in logarithmic phase in LB medium) under which there were no phenotypic difference between the wild type and pBClin15 cured strain as well as under stressful conditions under which there were phenotypic differences between the two strain (growth in LB medium supplemented with 0.5M-5g norfloxacin per ml).

2013-09-10 | E-MEXP-3894 | biostudies-arrayexpress

Transcription profiling of 3 Bacillus cereus strains in aerobic or CO2-bicarbonate atmospheres

Project description:Growth of 3 related Bacillus strains in a semi-defined medium in two different atmospheres (14% CO2 and high aeration)

2009-02-06 | E-MEXP-2036 | biostudies-arrayexpress

Characterization of PlcRa, a new PlcR-like regulator involved in oxidative stress response and cysteine metabolism during early stationary phase in Bacillus cereus

Project description:PlcR, the major virulence transcriptional regulator in the Bacillus cereus group belongs to the RNPP quorum sensor family and is activated through interaction with the secreted heptapeptide PapR7 used as a signalling molecule. We identified using modeling analysis a structural homologue of PlcR, PlcRa, in B. cereus. Whole-genome comparative microarrays identified 119 genes (threshold >3) differentially expressed at the onset of stationary phase in a plcRa mutant. phBC6A51 and phBC6A52 prophage genes were up-expressed in a plcRa mutant. Genes involved in oxidative stress response and in cysteine metabolism, including genes which belong to the CymR regulon, were down-regulated in a plcRa mutant. Accordingly, deletion of plcRa resulted in increased sensitivity to hydrogen peroxide and disulfide induced stresses. We also characterized a major target of PlcRa, the (BC2444) abrB2 gene, encoding a putative regulator belonging to AbrB family. PlcRa controlled CymR-regulated genes through AbrB2 and deletion of abrB2 gene resulted in increased sensitivity to hydrogen peroxide and disulfide induced stresses. In conclusion, we identified a new early stationary transcriptional regulator specific to B. cereus group members involved in oxidative stress response and CymR regulon activation. Two different comparisons were performed (both in biological duplo and Cy5-Cy3 dye-swapped) using 2 different time points: (1) B.cereus WT versus B. cereus plcRa overexpression strain at T1 (one hour after the onset of stationary phase) and T2 (two hours after the onset of stationary phase). (2) B.cereus WT versus B.cereus plcRa deletion strain at T1 and T2

2012-12-14 | E-GEOD-30514 | biostudies-arrayexpress

Transcriptome comparison between Bacillus cereus ATCC 14579 wildtype (WT), RpoN (Sigma 54) deletion mutant and the complemented strain.

Project description:RpoN was shown to have a pleiotropic role in different microorganisms. This study was performed to elucidate role in B. cereus. In this study we compared the transcriptomic profiles of the WT, rpoN mutant and the complemented strain in aerated and static growth conditions. Matrix is compatible with the Agilent-017343 array design. Aerated cultures were sampled at two time points, upon reaching OD (600 nm) values of 0.2 (t1) and 1.0 (t2), which corresponded to mid-exponential and transition growth phases, respectively. Statically grown cultures were sampled at OD=0.2 (600 nm) corresponding to exponential growth (exp/static). Two independent biological replicates were hybridized on the arrays with either 2 (WT) or 3 (Δrpon and ΔrpoN-comp) technical replicates for each biological replicate.

2015-07-28 | E-GEOD-65894 | biostudies-arrayexpress

Transcriptome comparison of E. coli W3110 and hns stpA hha and ydgT mutant cells

Project description:Transcriptome comparison of E.coli W3110 (wild type), hha, ydgT, hns, stpA, hha_ydgT double and hns_stpA double mutant cells

2013-04-04 | E-MEXP-3811 | biostudies-arrayexpress

Global gene expression analysis of Bacillus cereus to proanthocyanidin exposure

Project description:B. cereus RNA isolation were taken at OD 0.5 just before addition of 0.02 mg/mL EEC, and at 30 min of exposure. Water was added instead of EEC for control. Total RNA was isolated and we performed DNA microarray for genome-wide transcriptional analysis of B. cereus ATCC 14579 in the presence or absence of EEC. The microarray used in this study was custom-made B. cereus ATCC 14579 developed by Agilent Technologies (https://earray.chem.agilent.com/earray/). The B. cereus microarray design was based on the predicted chromosomal open reading frames (NCBI Accession No. AE016877). It targets the 5234 annotated CDS, three non-overlapping probes were designed, low-quality probes were eliminated, and finally 15242 could be spotted. Microarray data was normalized to 75th percentile and the difference was found to be significant by unpaired T-test (P = 0.05). Next, ratio changes of 2-fold (for up-regulated genes in the EEC stress condition) and 0.5-fold (for down-regulated genes in the EEC stress condition) were regarded as biologically significant. 596 were up-regulated and 495 were down-regulated. Bacillus cereus was selected at 0, 30 min exposure for RNA extraction and hybridization on Agilent microarrays.

2015-05-12 | E-GEOD-68767 | biostudies-arrayexpress

Transcription profiling of E. coli strain MC4100 in comparison to its otherwise isogenic ycgE::cat derivative to identify YcgE dependent genes

Project description:Genome-wide transcriptional profiling on microarray was performed for strain MC4100 in comparison to its otherwise isogenic ycgE::cat derivative. Strains were grown in LB at 28°C and cells were harvested at an OD (578nm) of 4.0.

2009-06-03 | E-MEXP-1872 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data