Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

60 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Kolsto-B. cereus Comparison of WT and BC4707 mutant during growth in logarithmic phase

ABSTRACT: Comparison of B. cereus ATCC14579 wild type and its isogenic BC4707 mutant under growth in logarithmic phase

ORGANISM(S): Bacillus cereus

SUBMITTER: Roger Simm

PROVIDER: E-MEXP-3537 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

BC4707 is a major facilitator superfamily multidrug resistance transport protein from Bacillus cereus implicated in fluoroquinolone tolerance.

Simm Roger R Vörös Aniko A Ekman Jaakko V JV Sødring Marianne M Nes Ingerid I Kroeger Jasmin K JK Saidijam Massoud M Bettaney Kim E KE Henderson Peter J F PJ Salkinoja-Salonen Mirja M Kolstø Anne-Brit AB

PloS one 20120516 5

Transcriptional profiling highlighted a subset of genes encoding putative multidrug transporters in the pathogen Bacillus cereus that were up-regulated during stress produced by bile salts. One of these multidrug transporters (BC4707) was selected for investigation. Functional characterization of the BC4707 protein in Escherichia coli revealed a role in the energized efflux of xenobiotics. Phenotypic analyses after inactivation of the gene bc4707 in Bacillus cereus ATCC14579 suggested a more spe ...[more]

PMID: 22615800

Similar Datasets

Kolsto-Bacillus cereus transcriptional response of ATCC14579 BC4707 mutant to bile salts stress

Project description:Analysis of the transcriptional response of B. cereus ATCC14579 BC4707 deletion mutant after 15 min bile salts stress

2012-01-31 | E-MEXP-3536 | biostudies-arrayexpress

Kolsto-B. cereus Transcriptional response of the B. cereus ATCC14579 wild type to bile salts stress

Project description:Comparison of the transcriptional response of B. cereus ATCC14579 before and after 15 min bile salts stress.

2012-01-31 | E-MEXP-3535 | biostudies-arrayexpress

Kolsto-Bacillus cereus comparison of wild type and bc4707 deletion mutant after Bile Salts stress

Project description:Comparison of wild type Bacillus cereus ATCC14579 and its isogenic bc4707 deletion mutant under logarithmic growth (OD=0.5)

2012-01-31 | E-MEXP-3529 | biostudies-arrayexpress

Transcription profiling by array of Burkholderia cenocepacia J2315 at the beginning of the stationary phase compared to mid-log phase

Project description:Burkholderia cenocepacia J2315 was grown at 37 degrees centigrade in LB broth to the beginning of stationary phase (18 hours incubation time). The expression profile was compared to cultures harvested in mid-log phase (7 hours incubation time).

2012-01-01 | E-MEXP-2790 | biostudies-arrayexpress

Transcriptome comparison of E. coli W3110 and hns stpA hha and ydgT mutant cells

Project description:Transcriptome comparison of E.coli W3110 (wild type), hha, ydgT, hns, stpA, hha_ydgT double and hns_stpA double mutant cells

2013-04-04 | E-MEXP-3811 | biostudies-arrayexpress

Transcription profiling by array of Burkholderia cenocepacia J2315 from the beginning of the stationary phase compared to mid-log phase

Project description:Burkholderia cenocepacia J2315 was grown in LB broth to the beginning of stationary phase. The expression profile was compared to cultures harvested in mid-log phase.

2012-01-01 | E-MEXP-2754 | biostudies-arrayexpress

Transcription profiling by array of Burkholderia cenocepacia J2315 grown at 20C or 37C

Project description:B. cenocepacia J2315 was grown on LB medium to mid-stationary phase at O.D. 0.5 at 150 rpm in a shaking incubator at two different temperatures: 37 degrees and 20 degrees centigrade.

2012-01-01 | E-MEXP-2789 | biostudies-arrayexpress

Gene transcription from the linear plasmid pBClin15 leads to cell lysis and extracellular DNA dependent aggregation of B. cereus ATCC 14579 in response to quinolone induced stress

Project description:Several members of the Bacillus cereus group of bacteria carry lysogenic phages of the family Tectiviridae. The Bacilus cereus reference strain (ATCC 14579) harbor a linear plasmid (pBClin15) that is highly similar to the genomes of the Tectiviruses. Production of active phages has however never been reported for pBClin15 and the role of pBClin15 in B. cereus physiology has been enigmatic. We have for the first time demonstrated an effect of pBClin15 on the physiology of B. cereus ATCC 14579 whereby the wild type is more sensitive to DNA damaging antibiotics compared to a pBClin15 cured strain. The microarray experiments in this study were designed to highlight transcriptional differences between the B. cereus ATCC 14579 wild type and a plasmid cured variant that potentially could explain the impact of pBClin15 on the B. cereus physiology. Microarray experiments were carried out under non-stressful conditions (growth in logarithmic phase in LB medium) under which there were no phenotypic difference between the wild type and pBClin15 cured strain as well as under stressful conditions under which there were phenotypic differences between the two strain (growth in LB medium supplemented with 0.5M-5g norfloxacin per ml).

2013-09-10 | E-MEXP-3894 | biostudies-arrayexpress

Transcription profiling by array of Burkholderia cenocepacia J2315 was exposed to heat stress and to stress form reactive oxygen species

Project description:B. cenocepacia J2315 was exposed to heat stress and to stress form reactive oxygen species. To expose the cultures to heat stress, cells were grown at 37ºC to an O.D. of 0.4 to 0.45 and then transferred into a different shaking incubator at 42.5ºC, incubated for 1 hour at 150 rpm and harvested. To expose cultures to oxidative stress by reactive oxygen species, cells were grown at 37ºC to an OD of 0.5. Then t butyl hydroperoxide or hydrogen peroxide solution were added to the culture at 0.001% and 0.15% final concentration. The culture was further incubated for 15 min and then harvested. The expression profiles were compared to cells grown in LB medium without exposure to stress.

2012-01-01 | E-MEXP-2791 | biostudies-arrayexpress

Transcription profiling by array of Burkholderia cenocepacia J2315 grown under low or atmospheric oxygen concentration conditions

Project description:B. cenocepacia J2315 was grown on LB medium to mid-stationary phase at OD 0.3 under full aeration and then transferred into a 50 ml centrifuge tube and placed upright into a CampyGen Compact (Oxoid, Basingstoke, UK) plastic pouch containing the gas generating paper sachet. The pouch was sealed immediately and then the cap of the centrifuge tube loosened to allow exchange of atmosphere between centrifuge tube and pouch. The culture was further incubated at 37 degrees centigrade and 150 rpm for 2-3 hours until it reached OD 0.5. The centrifuge tube was sealed before opening the pouch and then snap-cooled before harvest to minimise exposure to atmospheric oxygen and to ensure expression profiles reflect the lower oxygen concentration. The expression profile was compared to cells grown to OD 0.5 at atmospheric oxygen concentrations.

2012-01-01 | E-MEXP-2787 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data