Project description:Mice received TBI and allogeneic bone marrow transplantation. One group without and one group with T cells. On day 14 after transplantation mice were sacrificed and the intestinal tract was isolated. RNA was isolated from the intestinal tract of the two experimental groups.
Project description:Day 6 human monocyte derived dendritic cells were left unstimulated or matured for 2 days using the golden standard maturation cocktail currently used in clinical practice. Maturation induced changes in mRNA expression were determined by calculation of expression in mature DC relative to immature DC. Obtained gene expression raito's were compared to protein expression ratio's from the same batch of cells at the pathway level (proteomics data of this experiment is deposited in Human Proteinpedia, accession number pending).
Project description:To assess the effect of carbon nanotubes substrated on dendritic cell (DCs) properties, DCs were generated from human monocytes of healthy donors as previously described (Aldinucci A et al, 2010). In particular, isolated monocytes were cultured for 6 days in medium supplemented with GM-CSF (1000U/ml) and IL-4 (1000U/ml), in presence or absence of multi-walled carbon nanotubes (MWCNT); then DCs were activated by 24 hours of incubation with LPS (1ug/ml). RNA extracted from floating and CNT adherent DCs were then used for transcriptional profilingthen used
Project description:We have been interested whether peripheral tolerance can be restored by vaccination with antigen-presenting cells (APCs) loaded with apoptotic bodies. Dendritic cells (DCs) are powerful APCs that have a critical role in the initiation and progression of autoimmunity. We previously demonstrated that DCs loaded with apoptotic islet cells prevents experimental type 1 diabetes (T1D), an autoimmune disease caused by beta-cell destruction. The goal of this study is to characterize the molecular changes on gene expression -transcriptome- occurring in these DCs pulsed with apoptotic bodies. Cells from four different genetically identical NOD (Non-Obese Diabetic) mice were used to obtain DCs. DCs were loaded with apoptotic bodies from NIT-1 cell line (insulinoma from NOD mice). Unloaded DCs were used as control in four paired experiments. Moreover, transcriptome from NIT-1 apoptotic bodies was determined. Mouse Gene 1.0 ST Arrays from Affymetrix (28,853 genes) were hybridized and genes with a p-value <= 0.002, adjusted p-value <= 0.08 and fold change (FC) >= 1.38 were considered upregulated, and genes with FC <= -1.37 were considered downregulated. Results demonstrate that apoptotic cells engulfment promotes molecular changes in DCs towards tolerogenic features. Gene expression profile of DCs from NOD mice that captured NIT-1 apoptotic bodies showed a downregulation of genes involved in antigen presentation and differential expression of cytokine, chemokine, natural immunity and immunoregulation genes together with the presence of specific transcripts for islet autoantigens. Molecular changes of DCs caused by the uptake of apoptotic bodies are key factors to induce antigen-specific peripheral tolerance.
Project description:Aim of the experiment was to characterize the gene activation profile<br><br>induced within immature myeloid DCs by cell-cell contact with activated<br><br>invariant NKT cells under steady-state conditions.
Project description:Circadian transcriptional factor was overexpressed in the right muscle of a rat. Differential gene expression was analysed between the test and control muscle at two different timepoints (zT0 and zT12).
Project description:In order to establish the optimal conditions to study expression of genes of S. cerevisiae upon interaction with dendritic cells, Saccaromyces cerevisiae cells were cultured in presence of dendritic cells for 4 hours.<br>Dendritic cells were then treated with zymolase, the yeast cells recovered and lysed. RNA from lysed yeast cells was used for a transcription profiling analysis on Agilent arrays comparing internalized yeast with control yeast.
Project description:Comparison of osteoclastic differentiation pathway from DCs with the canonical differentiation pathway from monocytes by transcriptomic profiling
Project description:Analysis of PerR regulon. Global transcription profile of wild type GAS M1 (SF370) as compared to perR deletion mutant in mid- and late- log growth phases
Project description:Toll-like receptor (TLR) signalling activation by pathogens is critical to the induction of immune responses, and demands tight regulation. Chemokine ligand 2 (CCL2) secretion triggered by TLR4 or TLR8 engagement is strongly inhibited upon simultaneous activation of both TLRs in human monocyte-derived dendritic cells (MD-DC). Impaired CCL2 secretion occurs concomitantly to IL-12 up-regulation, being part of a complex regulatory circuit ensuring optimal Th type 1 polarization. Interestingly, triggering selected TLRs or their combinations differently affects nuclear factor-kB p65 activation and microRNA expression. To investigate in details such different modulation we performed a microarray profiling of MD-DCs stimulated by different TLRs agonist or their combination in three different donors. We found that CCL2 supplies an important immunomodulatory role to DCs, and may contribute to dictate the cytokine profile in Th type 1 responses induced by DCs.