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Transcription profiling of mouse mammary cell subpopulations isolated by flow cytometry, on the basis of staining with antibodies against CD45, CD49f, Sca-1 and the 33A10 antigen

ABSTRACT: We have carried out microarray-based gene expression analysis of cell subpopulations isolated by flow cytometry, on the basis of staining with antibodies against CD45, CD49f, Sca-1 and the 33A10 antigen, from freshly prepared mouse mammary glands. RNA isolated from cell populations was co-hybridised with control mouse RNA on to an in-house (Breakthrough Breast Cancer Centre) cDNA mouse microarray containing 13825 features (NIA 15K Mouse cDNA clone set). LIMMA analysis was used to analyse data and determine changes in RNA expression levels.

INSTRUMENT(S): Axon Instruments GenePix 4000B scanner

ORGANISM(S): Mus musculus

SUBMITTER: Anita Grigoriadis

PROVIDER: E-MEXP-423 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Regulator of G-protein signalling 2 mRNA is differentially expressed in mammary epithelial subpopulations and over-expressed in the majority of breast cancers.

Smalley Matthew J MJ Iravani Marjan M Leao Maria M Grigoriadis Anita A Kendrick Howard H Dexter Tim T Fenwick Kerry K Regan Joseph L JL Britt Kara K McDonald Sarah S Lord Christopher J CJ Mackay Alan A Ashworth Alan A

Breast cancer research : BCR 20070101 6

<h4>Introduction</h4>To understand which signalling pathways become deregulated in breast cancer, it is necessary to identify functionally significant gene expression patterns in the stem, progenitor, transit amplifying and differentiated cells of the mammary epithelium. We have previously used the markers 33A10, CD24 and Sca-1 to identify mouse mammary epithelial cell subpopulations. We now investigate the relationship between cells expressing these markers and use gene expression microarray an ...[more]

PMID: 18067675

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