Ontology highlight
ABSTRACT:
Microarray quality was determined by preliminary hybridizations with reference B31MI DNA labeled with both cy3 and cy5. The specificity of the arrays was assessed by cohybridization of cy-3 labeled DNA from strain B314, a B31 derivative lacking linear plasmids (Sadziene et al. 1993), with cy5-labeled B31MI DNA.
INSTRUMENT(S): GenePix 4000B [Axon Instruments]
ORGANISM(S): Borrelia burgdorferi
SUBMITTER: Ira Schwartz
PROVIDER: E-MEXP-735 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Journal of bacteriology 20060901 17
Clinical and murine studies suggest that there is a differential pathogenicity of different genotypes of Borrelia burgdorferi, the spirochetal agent of Lyme disease. Comparative genome hybridization was used to explore the relationship between different genotypes. The chromosomes of all studied isolates were highly conserved (>93%) with respect to both sequence and gene order. Plasmid sequences were substantially more diverse. Plasmids lp54, cp26, and cp32 were present in all tested isolates, an ...[more]