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Comparative genomic hybridization of 17 Borrelia burgdorferi isolates of varying pathogenicity and RST gene type to investigate genome plasticity


ABSTRACT: B. burgdorferi isolates differ in pathogenicity, and a correlation between RST type and pathogenicity was observed: the most invasive of the isolates are associated with RST1 and the least invasive with RST3 (Wang et al. 2001, Wang et al. 2002, Wormser et al. 1999). Comparative genome hybridization employing custom B. burgdorferi 70mer oligonucleotide microarrays representing all reference strain B31MI ORFs was used to assess genome plasticity, to reveal genes that are conserved and thus may encode essential functions and to reveal elements encoding potential virulence factors in 15 B. burgdorferi isolates (B479, B515, BL206, 297, B376, B379, BL224, B331, B348, B356, B408, B418, B500, JD1, N40) representing all three RST types. Total genomic DNA for each isolate was labeled with cy3 and hybridized simultaneously with cy5-labeled B31MI DNA as reference. Each hybridization was performed in duplicate.
Microarray quality was determined by preliminary hybridizations with reference B31MI DNA labeled with both cy3 and cy5. The specificity of the arrays was assessed by cohybridization of cy-3 labeled DNA from strain B314, a B31 derivative lacking linear plasmids (Sadziene et al. 1993), with cy5-labeled B31MI DNA.

INSTRUMENT(S): GenePix 4000B [Axon Instruments]

ORGANISM(S): Borrelia burgdorferi

SUBMITTER: Ira Schwartz 

PROVIDER: E-MEXP-735 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Comparative genome hybridization reveals substantial variation among clinical isolates of Borrelia burgdorferi sensu stricto with different pathogenic properties.

Terekhova Darya D   Iyer Radha R   Wormser Gary P GP   Schwartz Ira I  

Journal of bacteriology 20060901 17


Clinical and murine studies suggest that there is a differential pathogenicity of different genotypes of Borrelia burgdorferi, the spirochetal agent of Lyme disease. Comparative genome hybridization was used to explore the relationship between different genotypes. The chromosomes of all studied isolates were highly conserved (>93%) with respect to both sequence and gene order. Plasmid sequences were substantially more diverse. Plasmids lp54, cp26, and cp32 were present in all tested isolates, an  ...[more]

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