Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

38 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Medicago truncatula cell suspension cultures treated with invertase and/or S.meliloti lipopolysaccharide

ABSTRACT: This experiment studies the effect of different compounds on gene expression in M. truncatula cell cultures.

INSTRUMENT(S): ScanArray 4000 [PerkinElmer]

ORGANISM(S): Medicago truncatula

SUBMITTER: Verena Tellström

PROVIDER: E-MEXP-924 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

The lipopolysaccharide of Sinorhizobium meliloti suppresses defense-associated gene expression in cell cultures of the host plant Medicago truncatula.

Tellström Verena V Usadel Björn B Thimm Oliver O Stitt Mark M Küster Helge H Niehaus Karsten K

Plant physiology 20070112 2

In the establishment of symbiosis between Medicago truncatula and the nitrogen-fixing bacterium Sinorhizobium meliloti, the lipopolysaccharide (LPS) of the microsymbiont plays an important role as a signal molecule. It has been shown in cell cultures that the LPS is able to suppress an elicitor-induced oxidative burst. To investigate the effect of S. meliloti LPS on defense-associated gene expression, a microarray experiment was performed. For evaluation of the M. truncatula microarray datasets, ...[more]

PMID: 17220366

Similar Datasets

Transcription profiling of Medicago truncatula time series of flower and pod development

Project description:To study the development of flowers and pods in Medicago truncatula, eight stages of flower and pod development (designated F1-F4 and P1-P4, respectively) were chosen for tissue harvesting. As a common reference, young but already fully developed leaves (corresponding to the third-distant node from the apices) from plants grown in the field were selected. For each stage of flower and pod development, two biological replicates were harvested, one from plants grown in the greenhouse and one from plants grown in the field.

2005-05-31 | E-MEXP-143 | biostudies-arrayexpress

Transcription profiling of Medicago truncatula genes in roots in response to high (2 mM) and low (20 ?M) phosphate concentrations

Project description:Expression of Medicago truncatula genes in roots in response to high (2 mM) and low (20 µM) phosphate concentrations.

2005-06-30 | E-MEXP-238 | biostudies-arrayexpress

High-throughput pyrosequencing of miRNAs from Medicago truncatula nodules and roots

Project description:We have used deep sequencing of small RNAs from nodules and root apexes of the model legume Medicago truncatula, to identify 113 novel candidate miRNAs. These miRNAs (legume or Mt-specific) are encoded by 278 putative hairpin precursors in the M. truncatula genome. Several miRNAs are differentially expressed in nodules and root tips and large variety of targets could be predicted for these genes. Specific miRNA isoforms showed contrasting expression patterns in these tissues Keywords: Transcriptome analysis 3 samples examined: nodules, root tips, and root tips + NaCl

2009-07-07 | E-GEOD-15438 | biostudies-arrayexpress

Transcription profiling of Medicago truncatula 2HA and Jemmalong during somatic embryogenesis

Project description:The Medicago truncatula line 2HA has a 500-fold greater capacity to regenerate plants in culture by somatic embryogenesis than wild-type Jemalong. We have compared transcriptomes of tissue cultures from leaf explants of these two lines. We have used the Affymetrix Medicago Genome Array GeneChip to compare leaf explants of Medicago truncatula Jemalong and the superembryogenic line 2HA after two weeks of tissue culture on 10 ÂµM 1-naphthaleneacetic acid (NAA) and 4 ÂµM 6-benzylaminopurine (BAP) (P4 10:4) Experiment Overall Design: Leaf explants from the two genotypes were treated with 10:4 for two weeks; total RNA was extracted and used for hybridization to Affymetrix arrays

2008-06-16 | E-GEOD-8131 | biostudies-arrayexpress

myc cell types

Project description:cortical cell of non-mycorrhizal roots (cor), arbuscule-containing cells (arb) and non-arbuscule-containing cells (nac) of M. truncatula roots colonized with Glomus intraradices were collected by laser capture microdissection (LCM) and used for RNA extraction and Medicago microarray hybridisation

2011-10-05 | E-MEXP-3385 | biostudies-arrayexpress

High-throughput sequencing of small RNAs in Medicago truncatula

Project description:Medicago truncatula endogenous small RNAs The dataset contains Medicago truncatula Gaertn. cv. Jemalong endogenous small RNA sequences in the range 18-28 nucleotides. High-throughput Solexa/Illumina sequencing was carried out at the Sainsbury Laboratory, Norwich, UK. Please see www.illumina.com for details of the technology. Small RNA sequences were mapped to Medicago truncatula genome release 2.0 (http://www.medicago.org/genome/), the number of matches to the unfinished genome, if any, is recorded in the Series supplementary file GSE13761_sequence_annotations.txt.gz. Size fractionated small RNA from total RNA extracts was ligated to adapters, purified again and reverse transcribed. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.

2009-11-09 | E-GEOD-13761 | biostudies-arrayexpress

Transcription profiling of arbuscular mycorrhiza established at 4 weeks post inoculation between Medicago truncatula and Glomus mosseae

Project description:The transcriptome profile of arbuscular mycorrhiza established at 4 weeks post inoculation between Medicago truncatula and Glomus mosseae as well as between Medicago truncatula and Glomus intraradices is compared

2005-06-30 | E-MEXP-218 | biostudies-arrayexpress

Medicago truncatula and Macrophomina phaseolina interaction

Project description:This experiment was designed to study the interactions between Medicago truncatula and the charcoal rot pathogen Macrophomina phaeolina. Two-week-old plants grown in Magenta boxes supplied with 1/2 MS salt and 1% sucrose were inoculated with M. phaseolina covered wheat seeds, and roots were harvested at 24, 36 and 48 hours after inoculation. Control plants were mock inoculated with a sterile wheat seed, and roots were harvest 24 hours later. Pooled RNAs were used in the array experiment using Affymetrix GeneChip(r) Medicago Genome Array.

2012-05-15 | E-MEXP-3580 | biostudies-arrayexpress

Medicago truncatula wild-type and mutant plantlet roots treated with symbiotic lipochitooligosaccharides

Project description:This SuperSeries is composed of the following subset Series: GSE33636: Gene expression data from Medicago truncatula plantlet roots treated with symbiotic lipochitooligosaccharides (LCOs). GSE33637: Gene expression data from Medicago truncatula mutant plantlet roots treated with Myc-LCOs. Refer to individual Series

2012-05-30 | E-GEOD-33638 | biostudies-arrayexpress

Transcription profiling of Medicago trunculata leaf tissue over-expressing Legume Anthocyanin Production

Project description:Transcription profiling of leaf tissue from Medicago truncatula over-expressing Legume Anthocyanin Production.

2009-10-13 | E-MEXP-1854 | biostudies-arrayexpress