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Transcription profiling of Medicago truncatula time series of flower and pod development

ABSTRACT: To study the development of flowers and pods in Medicago truncatula, eight stages of flower and pod development (designated F1-F4 and P1-P4, respectively) were chosen for tissue harvesting. As a common reference, young but already fully developed leaves (corresponding to the third-distant node from the apices) from plants grown in the field were selected. For each stage of flower and pod development, two biological replicates were harvested, one from plants grown in the greenhouse and one from plants grown in the field.

ORGANISM(S): Medicago truncatula

SUBMITTER: Helge Kuester

PROVIDER: E-MEXP-143 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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EST sequencing and time course microarray hybridizations identify more than 700 Medicago truncatula genes with developmental expression regulation in flowers and pods.

Firnhaber Christian C Pühler Alfred A Küster Helge H

Planta 20050621 2

To evaluate the molecular mechanisms during pod and seed formation in legumes, starting with the development of reproductive organs, we constructed two cDNA libraries from developing flowers (MtFLOW) and pods including seeds (MtPOSE) of the model plant Medicago truncatula Gaertner. A total of 2,516 expressed sequence tags (ESTs) clustered into 1,776 nonredundant sequences (2k-set), which were annotated and assigned to functional classes. While about 30% of the ESTs encoded proteins of yet unknow ...[more]

PMID: 15968508

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