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Transcription profiling of Medicago truncatula treated with Sinorhizobium meliloti Nodulation factors at 6 and 48 h time points

ABSTRACT: This experiment studies the effect of purified Sinorhizobium meliloti Nodulation factors (Nod-factors, NF) on gene expression in the model legume Medicago truncatula.

INSTRUMENT(S): Scanning hardware

ORGANISM(S): Medicago truncatula

SUBMITTER: Helge Kuester

PROVIDER: E-MEXP-943 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification and expression regulation of symbiotically activated legume genes.

Küster Helge H Vieweg Martin F MF Manthey Katja K Baier Markus C MC Hohnjec Natalija N Perlick Andreas M AM

Phytochemistry 20061101 1

Legume plants are able to enter two different endosymbioses with soil prokaryotes and soil fungi, leading to nitrogen-fixing root nodules and to arbuscular mycorrhiza (AM), respectively. We applied in silico and microarray-based transcriptome profiling approaches to uncover the transcriptome of developing root nodules and AM roots of the model legume Medicago truncatula. Several hundred genes were found to be activated in different stages of either symbiosis, with almost 100 genes being co-induc ...[more]

PMID: 17081575

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Transcription profiling of Medicago truncatula time series of flower and pod development

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2005-05-31 | E-MEXP-143 | biostudies-arrayexpress

Transcription profiling of Medicago truncatula seeds at 14, 16, 20, 24, and 36 days post pollination

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Project description:Gene expression in mature, nitrogen-fixing root nodules was compared to gene expression in uninoculated roots.

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Project description:Comparison of gene expression in Medicago truncatula seed coats and endosperm, both against a reference sample from embryos.

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Transcription profiling of Medicago truncatula wild type and symbiotic mutant material

Project description:This experiment constitutes an expression profiling approach to identify genes differentially regulated during the symbiotic interaction between the model legume Medicago truncatula and the nitrogen-fixing bacterium Sinorhizobium meliloti. Macro- and microarrays containing 6144 probes were generated on the basis of three cDNA libraries dedicated to the study of root symbiotic interactions. The experiment performed on wild-type and symbiotic mutant material led to the identification of genes either up- or down-regulated at different stages of the nodulation process.

2004-06-28 | E-MEXP-129 | biostudies-arrayexpress

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Transcription profiling of developing wild type pea seeds and those expressing anti-ABA antibodies

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2008-09-01 | E-MEXP-1749 | biostudies-arrayexpress

Transcription profiling of developing pea cotyledons over-expressing VfAAP1

Project description:Nitrogen application to legume seeds regulates seed metabolism and composition. In order to improve nitrogen flux into the embryo, the Vicia faba amino acid permease VfAAP1 (Miranda et al. Amino acid permeases in developing seeds of Vicia faba L.: expression precedes storage protein synthesis and is regulated by amino acid supply. Plant J 2001 28: 61-72) was expressed in pea under control of the seed-specific LeB4 promotor (Bäumlein et al. Cis-analysis of a seed protein gene promoter: the conservative RY repeat CATGCATG within legumin box is essential for tissue-specific expression of a legumin gene. Plant J 1992 2: 233-239). Several transgenic lines have been generated. Mature seeds of the homozygous marker gene-free line AAP14/10 showed an increase in amino acid supply, seed nitrogen and protein content due to higher globulin fraction. The effect of VfAAP1 was tested under field conditions in two growing periods, 2005 and 2006, and the data could be confirmed. Over-expression of VfAAP1 interferes with storage protein metabolism and alters fluxes of nitrogen during seed growth. The influence of VfAAP1 on altered gene expression in developing cotyledons was analysed using a 6k-Oligo-microarray. Four developmental stages (18, 22, 26 and 30 DAP) from seeds, grown 2006, of the transgenic line AAP14/10 were hybridized against wildtype control.

2008-05-01 | E-MEXP-1557 | biostudies-arrayexpress

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