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Transcription profiling of Medicago truncatula wild type and symbiotic mutant material

ABSTRACT: This experiment constitutes an expression profiling approach to identify genes differentially regulated during the symbiotic interaction between the model legume Medicago truncatula and the nitrogen-fixing bacterium Sinorhizobium meliloti. Macro- and microarrays containing 6144 probes were generated on the basis of three cDNA libraries dedicated to the study of root symbiotic interactions. The experiment performed on wild-type and symbiotic mutant material led to the identification of genes either up- or down-regulated at different stages of the nodulation process.

ORGANISM(S): Medicago truncatula

SUBMITTER: Clare Gough 

PROVIDER: E-MEXP-129 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression profiling in Medicago truncatula identifies more than 750 genes differentially expressed during nodulation, including many potential regulators of the symbiotic program.

El Yahyaoui Fikri F   Küster Helge H   Ben Amor Besma B   Hohnjec Natalija N   Pühler Alfred A   Becker Anke A   Gouzy Jérôme J   Vernié Tatiana T   Gough Clare C   Niebel Andreas A   Godiard Laurence L   Gamas Pascal P  

Plant physiology 20041001 2

In this study, we describe a large-scale expression-profiling approach to identify genes differentially regulated during the symbiotic interaction between the model legume Medicago truncatula and the nitrogen-fixing bacterium Sinorhizobium meliloti. Macro- and microarrays containing about 6,000 probes were generated on the basis of three cDNA libraries dedicated to the study of root symbiotic interactions. The experiments performed on wild-type and symbiotic mutant material led us to identify a  ...[more]

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