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Transcription profiling of human wild type and deltaTOR-containing hepatocyte-like cells to compare total RNA and polysome-bound RNA populations upon hepatocytic differentiation

ABSTRACT: Comparison of Total RNA and Polysome-bound RNA populations in deltaTOR containing cells and control cells upon hepatocyitc differentiation.

ORGANISM(S): Homo sapiens

SUBMITTER: Deepak Kolippakkam

PROVIDER: E-MEXP-958 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mammalian target of rapamycin activation impairs hepatocytic differentiation and targets genes moderating lipid homeostasis and hepatocellular growth.

Parent Romain R Kolippakkam Deepak D Booth Garrett G Beretta Laura L

Cancer research 20070501 9

The mammalian target of rapamycin (mTOR) pathway, a major regulator of translation, is frequently activated in hepatocellular carcinomas. We investigated the effects of mTOR activation in the human HepaRG cells, which possess potent hepatocytic differentiation capability. Differentiation of HepaRG cells into functional and polarized hepatocyte-like cells correlated with a decrease in mTOR and Akt activities. Stable cell lines expressing an activated mutant of mTOR were generated. Sustained activ ...[more]

PMID: 17483347

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