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Transcription profiling of mouse naive T cells compared to regulatory T cells and activated T-cells induced to express Foxp3

ABSTRACT: CD4+CD25-CD62L+ naive T-cells were FACS isolated from wild type mouse lymph node. These were compared to regulatory T cells (Treg; CD4+C25+) and activated T-cells induced to express Foxp3 by treatment with rapamycin and LY294002 (Treg-like cells). RNA was extracted from duplicate cultures using RNA-Bee, labelled with the Affymetrix GeneChip Small Sample protocol and hybridised to Affymetrix Mouse 430 2.0 Arrays.

ORGANISM(S): Mus musculus

SUBMITTER: Jayne Dennis

PROVIDER: E-MIMR-1241 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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T cell receptor signaling controls Foxp3 expression via PI3K, Akt, and mTOR.

Sauer Stephan S Bruno Ludovica L Hertweck Arnulf A Finlay David D Leleu Marion M Spivakov Mikhail M Knight Zachary A ZA Cobb Bradley S BS Cantrell Doreen D O'Connor Eric E Shokat Kevan M KM Fisher Amanda G AG Merkenschlager Matthias M

Proceedings of the National Academy of Sciences of the United States of America 20080528 22

Regulatory T (Treg) cells safeguard against autoimmunity and immune pathology. Because determinants of the Treg cell fate are not completely understood, we have delineated signaling events that control the de novo expression of Foxp3 in naive peripheral CD4 T cells and in thymocytes. We report that premature termination of TCR signaling and inibition of phosphatidyl inositol 3-kinase (PI3K) p110alpha, p110delta, protein kinase B (Akt), or mammalian target of rapamycin (mTOR) conferred Foxp3 expr ...[more]

PMID: 18509048

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