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ATAC-seq of iPSC-derived hypothalamic arcuate-like neuron cells

ABSTRACT: We performed ATAC-seq on iPSC-derived hypothalamic arcuate-like neuron cells to identify putative regulatory elements. All samples were derived from the same individual and from the same differentiation/cell line but ATAC-seq was performed in 3 separate experiments (3 technical replicates).

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

SUBMITTER: Noboru Sakabe

PROVIDER: E-MTAB-10257 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Extensive pleiotropism and allelic heterogeneity mediate metabolic effects of IRX3 and IRX5.

Sobreira Débora R DR Joslin Amelia C AC Zhang Qi Q Williamson Iain I Hansen Grace T GT Farris Kathryn M KM Sakabe Noboru J NJ Sinnott-Armstrong Nasa N Bozek Grazyna G Jensen-Cody Sharon O SO Flippo Kyle H KH Ober Carole C Bickmore Wendy A WA Potthoff Matthew M Chen Mengjie M Claussnitzer Melina M Aneas Ivy I Nóbrega Marcelo A MA

Science (New York, N.Y.) 20210601 6546

Whereas coding variants often have pleiotropic effects across multiple tissues, noncoding variants are thought to mediate their phenotypic effects by specific tissue and temporal regulation of gene expression. Here, we investigated the genetic and functional architecture of a genomic region within the FTO gene that is strongly associated with obesity risk. We show that multiple variants on a common haplotype modify the regulatory properties of several enhancers targeting IRX3 and < ...[more]

PMID: 34083488

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