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RNA-seq of murine ES cells with Lima1 knockout, murine EpiS cells with Lima1 overexpression and human iPS cells with LIMA1 overexpression

ABSTRACT: In chimera assays, murine naïve embryonic stem (ES) cells usually produce better chimeras than the more primed epiblast-derived stem (EpiS) cells. Overexpression of the cytoskeleton-associated protein LIMA1/EPLIN in EpiS cells improves the rate of successful chimeras, while the knockout of LIMA1 in ES cells results in a metabolic state reminiscent of EpiS cells. To investigate the effects of LIMA1 we performed RNA-seq in Lima1 loss-of-function murine ES cells and in Lima1 gain-of-function murine EpiS cells and human induced pluripotent stem cells.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Fabian Groll

PROVIDER: E-MTAB-10301 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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