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Single-cell transcriptional landscape of human embryonic limb development - Visium

ABSTRACT: We performed spatial transcriptomic experiments using the 10x Visium assay, generating high-quality transcriptomic profiles for samples from PCW5 to PCW8

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Sarah Teichmann

PROVIDER: E-MTAB-10367 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A human embryonic limb cell atlas resolved in space and time.

Zhang Bao B He Peng P Lawrence John E G JEG Wang Shuaiyu S Tuck Elizabeth E Williams Brian A BA Roberts Kenny K Kleshchevnikov Vitalii V Mamanova Lira L Bolt Liam L Polanski Krzysztof K Li Tong T Elmentaite Rasa R Fasouli Eirini S ES Prete Martin M He Xiaoling X Yayon Nadav N Fu Yixi Y Yang Hao H Liang Chen C Zhang Hui H Blain Raphael R Chedotal Alain A FitzPatrick David R DR Firth Helen H Dean Andrew A Bayraktar Omer Ali OA Marioni John C JC Barker Roger A RA Storer Mekayla A MA Wold Barbara J BJ Zhang Hongbo H Teichmann Sarah A SA

Nature 20231206 8039

Human limbs emerge during the fourth post-conception week as mesenchymal buds, which develop into fully formed limbs over the subsequent months<sup>1</sup>. This process is orchestrated by numerous temporally and spatially restricted gene expression programmes, making congenital alterations in phenotype common<sup>2</sup>. Decades of work with model organisms have defined the fundamental mechanisms underlying vertebrate limb development, but an in-depth characterization of this process in humans ...[more]

PMID: 38057666

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