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Unknown,Transcriptomics,Genomics,Proteomics

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Identification of KIL1 targets via RNA-Seq in Zea mays

ABSTRACT: To understand how the NAC transcription factor KIL1 regulates age-induced senescence and cell death in maize silks, we need to get a genome-wide view on its downstream targets. We propose to compare the transcriptome profiles of GOF and LOF transgenic silk tissue with the profile of wild-type B104 silk. 1 cm of basal part of silk from rings 6-10 from plants harboring the dominant-negative repressor proSILK1:KIL1-SRDX, proSILK1:KIL1 overexpressing line, and wild type B104 will be harvested at 11 DASE. This will allow to compare and contrast the expression profiles of KIL1 LOF and GOF mutants with transcriptome data derived from wild type senescent silk.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Zea mays

SUBMITTER: Plant Systems Biology data submission

PROVIDER: E-MTAB-10850 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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KIL1 terminates fertility in maize by controlling silk senescence.

Šim Škov M Ria MR Daneva Anna A Doll Nicolas N Schilling Neeltje N Cubr A-Rad O Marta M Zhou Liangzi L De Winter Freya F Aesaert Stijn S De Rycke Riet R Pauwels Laurens L Dresselhaus Thomas T Brugi Re Norbert N Simmons Carl R CR Habben Jeffrey E JE Nowack Moritz K MK Nowack Moritz K MK

The Plant cell 20220701 8

Plant flowers have a functional life span during which pollination and fertilization occur to ensure seed and fruit development. Once flower senescence is initiated, the potential to set seed or fruit is irrevocably lost. In maize, silk strands are the elongated floral stigmas that emerge from the husk-enveloped inflorescence to intercept airborne pollen. Here we show that KIRA1-LIKE1 (KIL1), an ortholog of the Arabidopsis NAC (NAM (NO APICAL MERISTEM), ATAF1/2 (Arabidopsis thaliana Activation F ...[more]

PMID: 35608197

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